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Author Pelt-Verkuil, Elizabeth van, 1951-
Title Principles and technical aspects of PCR amplification / Elizabeth van Pelt-Verkuil, Alex van Belkum, John P. Hays.
Imprint [Dordrecht] : Springer, 2008.

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Subject Polymerase chain reaction -- Laboratory manuals.
Polymerase chain reaction -- Diagnostic use.
Gene Amplification.
Polymerase Chain Reaction.
Alt Name Belkum, Alex van.
Hays, John P.
Description 1 online resource (325 pages) : illustrations
Bibliography Note Includes bibliographical references and index.
Summary "This manual aims to provide the reader with a guide to the standard PCR technique and its many available modifications, with particular emphasis on the role of PCR techniques in the diagnostic laboratory (the central theme of this manual). Further, many important technical issues have been addressed, including types of PCR template material, PCR optimization, the analysis of PCR products, quality control, quantitative PCR and in situ PCR. The reader of this manual will be thoroughly informed about the fundamental principles of PCR and the true potential of PCR within clinical laboratory practice."--Jacket.
Contents The Polymerase Chain Reaction -- A Brief Comparison Between In Vivo DNA Replication and In Vitro PCR Amplification -- The PCR in Practice -- The Different Types and Varieties of Nucleic Acid Target Molecules -- PCR Primers -- Deoxynucleotide Triphosphates and Buffer Components -- Taq and Other Thermostable DNA Polymerases -- Important Considerations for Typical, Quantitative and Real-Time PCR Protocols -- Analysis of PCR Amplification Products -- Ensuring PCR Quality -- Laboratory Organisation, PCR Optimization and Controls -- Ensuring PCR Quality -- Quality Criteria and Quality Assurance -- Variants and Adaptations of the Standard PCR Protocol -- In Situ PCR Amplification (ISA) -- Major Considerations, Sample Processing and Applications.
Note Print version record.
ISBN 9781402062414
1402062419
9781402062407 (electronic bk.)
1402062400 (electronic bk.)
1281242373
9781281242372
OCLC # 261325797
Additional Format Print version: Pelt-Verkuil, Elizabeth van, 1951- Principles and technical aspects of PCR amplification. [Dordrecht] : Springer, 2008 9781402062407 1402062400 (DLC) 2007942548 (OCoLC)226279805