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LEADER 00000cam  2200637Ii 4500 
001    261325797 
003    OCoLC 
005    20181102030935.2 
006    m     o  d         
007    cr cn||||||||| 
008    081009s2008    ne a    ob    001 0 eng d 
019    227144503|a243780795|a261137261|a607293516|a607549800
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       |a1048131866|a1056349882|a1058113879 
020    9781402062414 
020    1402062419 
020    9781402062407|q(electronic bk.) 
020    1402062400|q(electronic bk.) 
020    1281242373 
020    9781281242372 
035    (OCoLC)261325797|z(OCoLC)227144503|z(OCoLC)243780795
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037    978-1-4020-6240-7|bSpringer|nhttp://www.springerlink.com 
040    GW5XE|beng|epn|erda|cGW5XE|dYDXCP|dEBLCP|dOCLCQ|dLDL|dDKU
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049    MAIN 
050  4 QP606.D46|bP45 2008eb 
072  7 PSAK|2bicssc 
072  7 SCI|x029000|2bisacsh 
082 04 572.8636|222 
082 04 572/.43|222 
100 1  Pelt-Verkuil, Elizabeth van,|d1951-|0http://id.loc.gov/
       authorities/names/n79115168 
245 10 Principles and technical aspects of PCR amplification /
       |cElizabeth van Pelt-Verkuil, Alex van Belkum, John P. 
       Hays. 
264  1 [Dordrecht] :|bSpringer,|c2008. 
300    1 online resource (325 pages) :|billustrations 
336    text|btxt|2rdacontent 
337    computer|bc|2rdamedia 
338    online resource|bcr|2rdacarrier 
340    |gpolychrome|2rdacc|0http://rdaregistry.info/termList/
       RDAColourContent/1003 
504    Includes bibliographical references and index. 
505 0  The Polymerase Chain Reaction -- A Brief Comparison 
       Between In Vivo DNA Replication and In Vitro PCR 
       Amplification -- The PCR in Practice -- The Different 
       Types and Varieties of Nucleic Acid Target Molecules -- 
       PCR Primers -- Deoxynucleotide Triphosphates and Buffer 
       Components -- Taq and Other Thermostable DNA Polymerases -
       - Important Considerations for Typical, Quantitative and 
       Real-Time PCR Protocols -- Analysis of PCR Amplification 
       Products -- Ensuring PCR Quality -- Laboratory 
       Organisation, PCR Optimization and Controls -- Ensuring 
       PCR Quality -- Quality Criteria and Quality Assurance -- 
       Variants and Adaptations of the Standard PCR Protocol -- 
       In Situ PCR Amplification (ISA) -- Major Considerations, 
       Sample Processing and Applications. 
520 1  "This manual aims to provide the reader with a guide to 
       the standard PCR technique and its many available 
       modifications, with particular emphasis on the role of PCR
       techniques in the diagnostic laboratory (the central theme
       of this manual). Further, many important technical issues 
       have been addressed, including types of PCR template 
       material, PCR optimization, the analysis of PCR products, 
       quality control, quantitative PCR and in situ PCR. The 
       reader of this manual will be thoroughly informed about 
       the fundamental principles of PCR and the true potential 
       of PCR within clinical laboratory practice."--Jacket. 
588 0  Print version record. 
650  0 Polymerase chain reaction|vLaboratory manuals.|0http://
       id.loc.gov/authorities/subjects/sh2010107437 
650  0 Polymerase chain reaction|xDiagnostic use.|0http://
       id.loc.gov/authorities/subjects/sh93001017 
650 12 Gene Amplification.|0https://id.nlm.nih.gov/mesh/D005784 
650 12 Polymerase Chain Reaction.|0https://id.nlm.nih.gov/mesh/
       D016133 
655  4 Electronic books. 
655  7 Laboratory manuals.|2fast|0http://id.worldcat.org/fast/
       1920776 
655  7 Laboratory manuals.|2lcgft|0http://id.loc.gov/authorities/
       genreForms/gf2014026120 
700 1  Belkum, Alex van.|0http://id.loc.gov/authorities/names/
       nb2008008805 
700 1  Hays, John P.|0http://id.loc.gov/authorities/names/
       nb2008008811 
776 08 |iPrint version:|aPelt-Verkuil, Elizabeth van, 1951-
       |tPrinciples and technical aspects of PCR amplification.
       |d[Dordrecht] : Springer, 2008|z9781402062407|z1402062400
       |w(DLC)  2007942548|w(OCoLC)226279805 
990    SpringerLink|bSpringer English/International eBooks 2008 -
       Full Set|c2018-11-02|yMaster record variable field(s) 
       change: 650|5OH1 
990    SpringerLink|bSpringer English/International eBooks 2008 -
       Full Set|c2018-10-31|yNew collection 
       springerlink.ebooks2008|5OH1 
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