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LEADER 00000cam  2201009Mi 4500 
001    868925744 
003    OCoLC 
005    20200911141810.9 
006    m     o  d         
007    cr cn||||||||| 
008    131219s2014    enka    ob    001 0 eng d 
019    866444908|a900557493|a900607511|a961587872|a962673257
       |a964591617|a992834692|a1162530870 
020    9781118495162|q(electronic bk.) 
020    1118495160|q(electronic bk.) 
020    9781118495148|q(electronic bk.) 
020    1118495144|q(electronic bk.) 
020    9781118495131|q(electronic bk.) 
020    1118495136|q(electronic bk.) 
020    9781306219426 
020    1306219426 
020    |z9780470672693 
020    |z0470672692 
028 01 EB00211204|bRecorded Books 
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049    MAIN 
050  4 RD598.5|b.M36 2014eb 
060  4 WG 120 
072  7 MED|x085000|2bisacsh 
082 04 617.4|a617.4/1059|a617.41059|223 
245 00 Manual of research techniques in cardiovascular medicine /
       |cedited by Hossein Ardehali, Roberto Bolli, Douglas W. 
       Losordo. 
264  1 Chichester, West Sussex ;|aHoboken :|bWiley,|c2014. 
300    1 online resource (473 pages) :|billustrations 
336    text|btxt|2rdacontent 
337    computer|bc|2rdamedia 
338    online resource|bcr|2rdacarrier 
504    Includes bibliographical references and index. 
505 0  Cover; Dedication; Title page; Copyright page; Contents; 
       List of Contributors; Preface; About the Companion 
       Website; Part 1: Electrophysiology; 1: Measurement of 
       calcium transient ex vivo; Introduction; Preparation and 
       fluorescent indicator loading; Optical setup; Calibration;
       Analysis and interpretation; Set-by-step procedure; 
       References; 2: Confocal imaging of intracellular calcium 
       cycling in isolated cardiac myocytes; Introduction; 
       Materials and methods; Chamber, electrical stimulation, 
       and superfusate delivery apparatus; Confocal microscope 
       system. 
505 8  Cell loading of fluorescent Ca2+ indicatorsExperimental 
       approach; Data analyses; Weaknesses and strengths of the 
       method and alternative approaches; Conclusions; 
       References; 3: Generating a large animal model of 
       persistent atrial fibrillation; Introduction; Insights 
       from tachypacing-induced AF models; Protocol; Generating a
       tachypacing-induced AF model; Pacing protocols; 
       Ventricular rate during AF: to ablate or not to ablate; 
       Strengths and weaknesses of the model; Complications and 
       alternative approaches to study AF; Potential 
       complications; Alternative AF models; Conclusion; 
       Acknowledgement. 
505 8  Choices to make before an experimentAP recording 
       procedure; Frequent problems; AP analysis; Alternative 
       approaches and extensions; Weaknesses and strengths; 
       Conclusion; References; 6: Patch-clamp recordings from 
       isolated cardiac myocytes; Introduction; Theory of patch-
       clamp recording; Cell-attached configuration; Whole-cell 
       recording configuration; Inside-out excised patch 
       configuration; Outside-out excised patch configuration; 
       Instrumentation for patch-clamp recordings; Experimental 
       aspects of patch-clamp recordings; Gigaseal formation; 
       Whole-cell recordings. 
505 8  Whole-cell voltage-clamp protocols, data acquisition, and 
       analysisConclusions and future directions; References; 7: 
       Optical mapping of the heart; Introduction; Protocols; 
       Experimental set-up; Tissue preparations (Figure 7.2); 
       Staining and image acquisition; Data processing and 
       analysis; Representative results; Alternative approaches; 
       Weaknesses and strengths of method; Conclusions; 
       References; Part 2: Isolation and Maintenance of Primary 
       Stem Cells; 8: Isolation of colony-forming endothelial 
       progenitor cells; Introduction; Materials; Laboratory 
       reagents; Laboratory equipment; Methods. 
520    While some research methods or techniques are applicable 
       in several areas of medicine, research in cardiovascular 
       diseases requires knowledge of an increasing array of 
       procedures, techniques and measurements that are highly 
       specialized and unique to this area of investigation. 
       Edited by senior clinical investigators who are recognized
       leaders in cardiovascular medicine worldwide, this book 
       provides readers with a comprehensive, practical "how-to-
       do-it" review of best-practice techniques for 
       cardiovascular research. 
546    English. 
588 0  Online resource; title from PDF title page (ebrary, viewed
       January 16, 2014). 
650  0 Angioplasty. 
650  0 Coronary heart disease|xTreatment. 
650  0 Tachycardia|xTreatment. 
650  0 Transluminal angioplasty. 
650  7 MEDICAL|xSurgery|xGeneral.|2bisacsh 
650  7 Angioplasty.|2fast|0(OCoLC)fst00808825 
650  7 Coronary heart disease|xTreatment.|2fast
       |0(OCoLC)fst00879569 
650  7 Transluminal angioplasty.|2fast|0(OCoLC)fst01154851 
650 12 Cardiovascular Diseases. 
650 22 Cardiology|xmethods. 
650 22 Models, Cardiovascular. 
650 22 Research Design. 
700 1  Ardehali, Hossein.|4edt 
700 1  Bolli, Roberto.|4edt 
700 1  Losordo, Douglas.|4edt 
776 08 |iPrint version:|tManual of research techniques in 
       cardiovascular medicine.|z9780470672693|z0470672692|w(DLC)
       2013017936 
990    Rittenhouse Books, Inc.|bRittenhouse R2 Digital Library 
       (Patron Driven Acquisitions)|c2020-09-11|yCustom text 
       change|5MHT 
990    Rittenhouse Books, Inc.|bRittenhouse R2 Digital Library 
       (Patron Driven Acquisitions)|c2020-09-03|yNew collection 
       rittenhouse.ebookspda|5MHT 
990    John Wiley and Sons|bWiley Frontlist All Obook 2014 to 
       present|c2020-01-18|yNew collection wiley.front2014|5OH1 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2018-11-02|yMaster record variable field(s) change: 650
       |5OXB 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2018-11-02|yMaster record variable field(s) change: 650
       |5MAL 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2018-11-02|yMaster record variable field(s) change: 650
       |5MTU 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2018-04-13|yMaster record variable field(s) change: 650
       |5OXB 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2018-04-13|yMaster record variable field(s) change: 650
       |5MAL 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2018-04-13|yMaster record variable field(s) change: 650
       |5MTU 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2018-03-09|yAdded to collection pqebk.acadcomplete - 
       Removed from collection pqebk.acadcomplete|5MTU 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2018-03-09|yAdded to collection pqebk.acadcomplete - 
       Removed from collection pqebk.acadcomplete|5MAL 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2018-03-09|yAdded to collection pqebk.acadcomplete - 
       Removed from collection pqebk.acadcomplete|5OXB 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2017-09-22|yMaster record variable field(s) change: 650
       |5OXB 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2017-09-22|yMaster record variable field(s) change: 650
       |5MTU 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2017-09-22|yMaster record variable field(s) change: 650
       |5MAL 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2017-09-15|yMaster record variable field(s) change: 650
       |5MAL 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2017-09-15|yMaster record variable field(s) change: 650
       |5OXB 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2017-09-15|yMaster record variable field(s) change: 650
       |5MTU 
990    ProQuest ebrary|bebrary Academic Complete|c2017-08-11
       |yRemoved from collection ebrary.ebooks|5MTU 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2017-08-11|yCustom text change|5MTU 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2017-07-28|yAdded to collection pqebk.acadcomplete - 
       Removed from collection pqebk.acadcomplete|5MAL 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2017-06-30|yMaster record variable field(s) change: 650
       |5OXB 
990    ProQuest ebrary|bebrary Academic Complete|c2017-06-30
       |yMaster record variable field(s) change: 650|5MTU 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2017-06-23|yMaster record variable field(s) change: 650
       |5OXB 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2017-07-21|yAdded to collection pqebk.acadcomplete|5MAL 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2017-08-05|yAdded to collection pqebk.acadcomplete|5MTU 
990    ProQuest ebrary|bebrary Academic Complete|c2017-06-23
       |yMaster record variable field(s) change: 650|5MTU 
990    ProQuest Ebook Central|bEbook Central Academic Complete
       |c2017-06-16|5OXB 
990    ProQuest ebrary|bebrary Academic Complete|c2017-04-20|5MTU
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