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Title DNA repair protocols : prokaryotic systems / edited by Pat Vaughan.
Imprint Totowa, N.J. : Humana Press, 2000.

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Series Methods in molecular biology ; 152
Methods in molecular biology (Clifton, N.J.) ; v. 152.
Subject DNA repair -- Laboratory manuals.
DNA Repair.
Prokoryotic Cells -- cytology
Alt Name Vaughan, Pat.
Description 1 online resource (x, 209 pages) : illustrations
Bibliography Note Includes bibliographical references and index.
Contents pt. 1. Assays to detect and quantitate DNA repair activity -- Repair of A/G and A/8-oxoG mismatches by mutY adenine DNA glycosylase -- Assays for the repair of oxidative damage by formanidopyrimidine glycosylase (Fpg) and 8-oxoguanine DNA glycosylase (OGG-1) -- Detection and quantitation of uracil DNA glycosylase activity -- Detection of DNA deoxyribophosphodiesterase activity -- O⁶-alkyguanine-DNA alkyltransferase assay -- The E. coli vsr endonuclease: assaying activity in vitro and in vivo -- Coupling of DNA helicase function to DNA strand exchange activity -- Detection and quanitation of recBCD enzymer (exonuclease V) activity -- Holiday junction branch migration and resolution: RuVA, RuvB, and RuvC from the hyperthermophile thermotoga maritima -- MutS-DNA interacitons and DNase protection analysis with surface plasmon resonance -- pt. 2. Use of DNA repair enzymes as tools in molecular biology -- Flexible genetic engineering using RecA protein -- Interspecies recombination and mismatch repair: generation of mosaic genes and genomes -- The use of immobilized mismatch binding protein in mutation/SNP detection -- Use of uracil DNA glycosylase in the detection of known DNA mutations and polymorphisms: glycosylase-mediated pholymorphism detection (GMPD-check) -- Use of uracil DNA glycosylase in scanning for unknown DNA mutations and polymorphisms: glycosylase-mediated polymorphism detection (GMPD-scan) -- The use of resolvases T4 endonuclease VII and T7 endonuclease I in mutation detection.
Note Print version record.
Summary DNA repair has assumed a new importance with the discovery that malfunctioning of the DNA repair pathways in humans can lead to many disease states. In DNA Repair Protocols: Prokaryotic Systems, well-versed investigators describe in step-by-step detail a wide range of DNA repair activities, from single act-alone repair proteins to complex repair systems. These practical protocols not only detail the various repair activities found in cells, but also demonstrate the use of DNA repair proteins and systems as reagents in molecular biology and biotechnology. The techniques described here include mutation and polymorphism detection, which are useful in the search for disease genes and drug response genes, as well as for breeding and trait selection in animals and plants. Each readily reproducible protocol is presented by a hands-on expert in sufficient detail to ensure robust experimental results and is supplemented by chapter introductions, as well as notes offering a wealth of interesting and useful information. Compact and highly practical, DNA Repair Protocols: Prokaryotic Systems provides expert guidance to both the DNA repair researcher studying the fundamental aspects of DNA repair and the applied researcher in human genetics and biotechnology.
Note English.
ISBN 1592590683 (Springer e-ISBN)
9781592590681 (Springer e-ISBN)
0585354332 (electronic bk.)
9780585354330 (electronic bk.)
089603643X (alk. paper)
9780896036437 (alk. paper)
ISBN/ISSN 10.1385/1592590683.
OCLC # 47009799
Additional Format Print version: DNA repair protocols. Totowa, N.J. : Humana Press, 2000 089603643X (DLC) 99086507 (OCoLC)43095748
Table of Contents
Pt. IAssays to Detect and Quantitate DNA Repair Activity 
 1Repair of A/G and A18-oxoG Mismatches by MutY Adenine DNA Glycosylase / A.-Lien Lu3
 2Assays for the Repair of Oxidative Damage by Formamidopyrimidine Glycosylase (Fpg) and 8-Oxoguanine DNA Glycosylase (OGG-1) / Amanda J. Watson, Geoffrey P. Margison17
 3Detection and Quantitation of Uracil DNA Glycosylase Activity / Geraldine M. O'Grady33
 4Detection of DNA Deoxyribophosphodiesterase Activity / Margarita Sandigursky, William A. Franklin39
 5O[superscript 6]-Alkylguanine-DNA Alkyltransferase Assay / Amanda J. Watson, Geoffrey P. Margison49
 6The E. coli Vsr Endonuclease: Assaying Activity In Vitro and In Vivo / Claire G. Cupples, Georgina MacIntyre63
 7Coupling of DNA Helicase Function to DNA Strand Exchange Activity / Frank G. Harmon, Stephen C. Kowalczykowski75
 8Detection and Quantitation of RecBCD Enzyme (Exonuclease V) Activity / Douglas A. Julin91
 9Holliday Junction Branch Migration and Resolution: RuvA, RuvB, and RuvC from the Hyperthermophile Thermotoga maritima / Susana Gonzalez, James G. Wetmur107
 10MutS-DNA Interactions and DNase Protection Analysis with Surface Plasmon Resonance / Peter Brooks119
Pt. IIUse of DNA Repair Enzymes as Tools in Molecular Biology 
 11Flexible Genetic Engineering Using RecA Protein / Lance J. Ferrin135
 12Interspecies Recombination and Mismatch Repair: Generation of Mosaic Genes and Genomes / Ivan Matic, Francois Taddei, Miroslav Radman149
 13The Use of Immobilized Mismatch Binding Protein in Mutation/SNP Detection / Robert Wagner, Alan Dean159
 14Use of Uracil DNA Glycosylase in the Detection of Known DNA Mutations and Polymorphisms: Glycosylase-Mediated Polymorphism Detection (GMPD-Check) / Pat Vaughan169
 15Use of Uracil DNA Glycosylase in Scanning for Unknown DNA Mutations and Polymorphisms: Glycosylase-Mediated Polymorphism Detection (GMPD-Scan) / Pat Vaughan179
 16The Use of Resolvases T4 Endonuclease VII and T7 Endonuclease I in Mutation Detection / Jeffrey J. Babon, Matthew McKenzie, Richard G. H. Cotton187

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