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Title PCR technology : current innovations / edited by Thomas Weissenteiner, Hugh G. Griffin, and Annette Griffin.
Imprint Boca Raton, Fla. : CRC Press, [2004]
Edition Second edition.

Subject Polymerase chain reaction -- Methodology.
Polymerase Chain Reaction -- methods.
Alt Name Weissensteiner, Thomas.
Griffin, Hugh G.
Griffin, Annette M.
Description 392 pages : illustrations ; 26 cm
Edition Second edition.
Bibliography Note Includes bibliographical references and index.
ISBN 0849311845 (alk. paper)
OCLC # 52688133
Table of Contents
Ch. 1Extraction and Amplification of Ancient DNA / Anne C. Stone5
Ch. 2Long PCR from Damaged DNA / Bernard Fromenty, Philippe Schmitt, Dominique Pessayre11
Ch. 3Quantitative mRNA Analysis in Small Cell Samples by RT-PCR and Flow Cytometry / Veronika Stemme, Sten Stemme23
Ch. 4Microdissection of Single or Small Numbers of Cells for Analyses of DNA and RNA by PCR / Rose G. Mage, Robert F. Bonner, Harold Obiakor29
Ch. 5Pre-PCR Processing Strategies / Peter Radstrom, Maria Lovenklev, Petra Wolffs, Charlotta Lofstrom, Rickard Knutsson37
Ch. 6Novel PCR-Enhancing Compounds and Their Modes of Action / Raj Chakrabarti51
Ch. 7Optimizing PCR with the Aid of Experimental Design / Thomas Weissensteiner65
Ch. 8Economic Fluorescent Labeling of PCR Products for Microsatellite- and Single-Stranded Conformation Polymorphism (SSCP) Analysis / Markus Schuelke79
Ch. 9Universal Restriction Site-Free Cloning Method Using Chimeric Primers / Guo Jun Chen87
Ch. 10High-Throughput Genotyping and the PCR / Kalim U. Mir, Jiannis Ragoussis97
Ch. 11High-Throughput Genetic Analysis through Multiplexed PCR and Multicapillary Electrophoresis / John M. Butler, Peter M. Vallone111
Ch. 12The GOOD Assay: A Purification-Free Assay for Genotyping by MALDI Mass Spectrometry / Jorg Tost, Ramon Kucharzak, Doris Lechner, Ivo Glynne Gut121
Ch. 13Primer Design for Large-Scale Multiplex PCR and Arrayed Primer Extension (APEX) / Maido Remm, Ants Kurg, Andres Metspalu131
Ch. 14Surface Plasmon Resonance Based Biosensor Technology for Real-Time Detection of PCR Products / Giordana Feriotto, Roberto Gambari141
Ch. 15Fluorescent Amplified Fragment Length Polymorphism (FAFLP) Genotyping / Catherine Arnold155
Ch. 16Global Analysis of DNA Allelic Variations (GADAV) by Specific Enrichment of Mismatches and Selective Amplification of Heterohybrids / Xinghua Pan, Sherman M. Weissman163
Ch. 17Quantitative DNA-Methylation Analysis by the Bisulfite Conversion Method / Osman El-Maarri, Manfred Kuepper, Johannes Oldenburg, Jorn Walter175
Ch. 18Pyrosequencing Technology / Pal Nyren, Tommy Nordstrom, Baback Gharizadeh187
Ch. 19Octamer-Primed Cycle Sequencing / Anelia I. Kraltcheva, Susan H. Hardin197
Ch. 20In Situ Amplification of cDNA / Gerard J. Nuovo205
Ch. 21Detection of a Single-Copy Sequence on Human Metaphase by Cyclic PRINS / Kirsten Mennicke, Sven Opitz, Eberhard Schwinger215
Ch. 22Meaningful Quantification of mRNA Using Real-Time PCR / Stephen A. Bustin225
Ch. 23Absolute Quantification of Specific Nucleic Acids by RT-PCR Using a Nonlinear Mathematical Model for Data Analysis / Huong L. Vu, Serge Troubetzkoy, Vu Q. Nguyen, Michael W. Russell, Jiri Mestecky235
Ch. 24Single-Molecule PCR - Basic Protocols and Applications / Paul H. Dear245
Ch. 25Gene Expression and Discovery: Application of a Genome-Wide Approach / Timothy A. Lohret, Karen M. Kelly259
Ch. 26The Detection of Differential Gene Expression Changes Using SAGE (Serial Analysis of Gene Expression) / Jill Powell267
Ch. 27Applications of Differential Display in Infectious Diseases / Irene Bosch, Rajas V. Warke, Marcia F. Fournier, David Magee, Heather Melichar277
Ch. 28Restriction Endonucleolytic Analysis of Differentially Expressed Sequences: READS / Yerramilli V. B. K. Subrahmanyam, Sherman M. Weissman287
Ch. 29Suppression Subtractive Hybridization PCR / Wan Ji, Li Cai299
Ch. 30PCR-Based Techniques for Cloning an Unknown DNA Fragment Adjacent to a Known Integrant / Eric Ka-Wai Hui, Szecheng J. Lo313
Ch. 31Chromosome Walking by Inverse PCR / Jorge A. Garces, Selwyn A. Williams, Ray H. Gavin323
Ch. 32Long Universal Fast Walking / Kyl V. Myrick329
Ch. 33Cloning of Unknown Virus Sequences by DNase Treatment and Sequence-Independent Single Primer Amplification / Tobias Allander, Suzanne U. Emerson, Robert H. Purcell, Jens Bukh337
Ch. 34Whole Genome Amplification from Single Cells and Minute DNA Samples / Dagan Wells, Mercedes G. Bermudez349
Ch. 35Primer Extension Preamplification (PEP) of Single Cells: Efficiency and Bias / Xiao Yan Zhong, Ying Li, Wolfgang Holzgreve, Sinuhe Hahn361
Ch. 36In Vitro Evolution through PCR-Mediated Mutagenesis and Recombination / Sarah B. Faulkner, Heather A. Syrett, Eric A. Davidson, Andrew D. Ellington371

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