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EBOOK
Author Jorgensen, James H.
Title Manual of clinical microbiology.
Imprint [Place of publication not identified] ASM Press 2015.

Subject Medical microbiology -- Handbooks, manuals, etc.
Diagnostic microbiology -- Handbooks, manuals, etc.
Clinical Laboratory Techniques.
Biology.
Biological Science Disciplines.
Investigative Techniques.
Analytical, Diagnostic and Therapeutic Techniques and Equipment.
Natural Science Disciplines.
Disciplines and Occupations.
Microbiological Techniques.
Microbiology.
Alt Name Jorgensen, James H.,
Jorgensen, James H.,
Pfaller, Michael A.,
Carroll, Karen C.,
Carroll, Karen C.,
Pfaller, Michael A.,
American Society for Microbiology,
Description 1 online resource
Note English.
Contents Half Title -- Title Page -- Copyright -- Contents -- Editorial Board -- Contributors -- Preface -- Author and Editor Conflicts of Interest -- section I DIAGNOSTIC STRATEGIES AND GENERAL TOPICS -- 1 Introduction to the 11th Edition of the Manual of Clinical Microbiology -- 2 Microscopy -- TECHNICAL BACKGROUND AND DEFINITION OF TERMS -- Aberration -- Contrast -- Depth of Field and Depth of Focus -- Immersion Fluid (Immersion Oil) -- Kohler Illumination -- Mechanical Tube Length -- Numerical Aperture -- Refractive Index (Index of Refraction) -- Resolution (Resolving Power) -- Working Distance -- SIMPLE MICROSCOPE -- COMPOUND MICROSCOPE -- Optical Train -- Field Diaphragm -- Substage Condenser -- Objectives -- DARK-FIELD MICROSCOPY -- PHASE-CONTRAST MICROSCOPY -- FLUORESCENCE MICROSCOPY -- LINEAR MEASUREMENTS (MICROMETRY) -- PHOTOMICROSCOPY -- CARE AND USE OF THE MICROSCOPE -- ERGONOMICS -- CONCLUSIONS -- REFERENCES -- 3 Laboratory Detection of Bacteremia and Fungemia -- ASSESSMENT OF METHODS FOR DETECTING MICROORGANISMS IN BLOOD -- DIAGNOSTIC IMPORTANCE -- PROGNOSTIC IMPORTANCE -- CRITICAL FACTORS -- Volume of Blood Cultured -- Number of Blood Cultures -- Dilution of Blood -- Anticoagulants -- Agitation -- Medium and Additives -- SPECIMEN COLLECTION -- Skin Disinfection -- Number and Timing of Cultures -- INTERPRETATION OF BLOOD CULTURE RESULTS -- LABORATORY DETECTION-CULTURE-BASED METHODS -- Manual Blood Culture Systems -- Lysis-Centrifugation -- Automated Blood Culture Systems -- BacT/Alert -- Bactec 9000 Series -- VersaTREK Blood Culture System -- Pediatric Blood Culture Bottles -- LABORATORY DETECTION-NON-CULTURE-BASED METHODS -- Surrogate Markers for Sepsis -- Nucleic Acid Amplification Methods -- RAPID IDENTIFICATION OF MICROBIAL ISOLATES -- Peptide Nucleic Acid-Fluorescent In Situ Hybridization -- Nucleic Acid Amplification Tests.
Antigen Detection -- MALDI-TOF (MS) -- DIRECT RAPID ANTIMICROBIAL SUSCEPTIBILITY TESTING FROM BLOOD CULTURE BOTTLES -- QUALITY AUDITS AND BENCHMARKS -- SUMMARY -- REFERENCES -- 4 Systems for Identification of Bacteria and Fungi -- ORGANISM IDENTIFICATION SYSTEMS -- Overview of Methods and Mechanisms of Identification -- System Construction -- CRITERIA FOR SELECTING INSTRUMENTED SYSTEMS -- EVALUATING AN INSTRUMENT OR SYSTEM -- Validating a New Instrument or Method -- LIMITATIONS OF MICROORGANISM IDENTIFICATION SYSTEMS -- PHENOTYPIC IDENTIFICATION SYSTEMS -- Automated Instruments (Vitek, Phoenix, MicroScan, TREK) -- Manual Kits, Assays, and Nonautomated Platforms -- PHENOTYPE-INDEPENDENT METHODS FOR IDENTIFICATION -- PROTEOMIC IDENTIFICATION SYSTEMS: MALDI-TOF MS (BASED IN PART ON REFERENCE 31) -- GENOTYPIC IDENTIFICATION SYSTEMS -- DNA Target Sequencing -- PCR Electrospray Ionization Mass Spectrometry -- REFERENCES -- 5 Automation and Design of the Clinical Microbiology Laboratory -- GEOGRAPHY OF THE LABORATORY -- LOCATION OF TESTING -- INFECTIOUS DISEASE TESTING IN THE CHEMISTRY AND CORE LABORATORIES -- PREANALYTICAL MICROBIOLOGY -- STAFFING MODELS -- STAFF TRAINING LEVEL -- WORKFLOW -- Batch Versus Immediate Testing -- Role of Process Improvement -- LABORATORY AUTOMATION: HISTORICAL PERSPECTIVES -- CURRENT SYSTEMS FOR MICROBIOLOGY LAB AUTOMATION -- Organism Identification and Susceptibility Testing -- Molecular Automation -- Automated Specimen Processing -- Total Laboratory Automation -- Digital Imaging -- Limitations of the Systems -- Criteria for Evaluation and Selection of an Automation System -- FUTURE PERSPECTIVES -- SUMMARY -- REFERENCES -- 6 Molecular Microbiology -- NONAMPLIFIED NUCLEIC ACID PROBES -- AMPLIFIED NUCLEIC ACID TECHNIQUES -- SIGNAL AMPLIFICATION TECHNIQUES -- bDNA Assays -- Hybrid Capture Assays -- Cleavase-Invader Technology.
TARGET AMPLIFICATION TECHNIQUES -- Polymerase Chain Reaction -- Reverse Transcriptase PCR -- Nested PCR -- Multiplex PCR -- Real-Time (Homogeneous, Kinetic) PCR -- Digital PCR -- Transcription-Based Amplification Methods -- Strand Displacement Amplification -- Loop-Mediated Amplification -- Helicase-Dependent Amplification -- POSTAMPLIFICATION DETECTION AND ANALYSIS -- Gel Analysis -- Capillary Electrophoresis -- Colorimetric Microtiter Plate Systems -- Allele-Specific Hybridization -- Nucleic Acid Sequencing -- Hybridization Arrays -- Mass Spectrometry -- QUANTITATIVE METHODS -- AUTOMATION AND INSTRUMENTATION -- CURRENT APPLICATIONS -- Initial Diagnosis -- Identification of Bacteria and Fungi by Nucleic Acid Sequencing -- Disease Prognosis -- Duration of and Response to Therapy -- LABORATORY PRACTICE -- Specimen Collection, Transport, and Processing -- Contamination Control -- Quality Control and Assurance -- Reporting and Interpretation of Results -- Regulatory and Reimbursement Issues -- Credentials -- FUTURE DIRECTIONS -- REFERENCES -- 7 Immunoassays for Diagnosis of Infectious Diseases -- HISTORIC PERSPECTIVE ON IMMUNOASSAY DEVELOPMENT -- DEFINITION OF TERMS -- GENERAL CONCEPTS OF ASSAY DESIGN -- Categorization of Serologic Assays -- Determination of Assay Performance Characteristics -- Screening versus Diagnostic Assays -- GENERAL CONCEPTS OF SEROLOGIC ASSAYS -- Basic Principles of Immunology -- Specimen Requirements -- Use of Serologic Analysis as a Measure of Protective Immunity -- Use of Serologic Analysis as a Measure of Recent versus Remote Infection -- Quantification of EIAs -- SPECIFIC IMMUNOASSAYS -- Precipitation Reactions -- Agglutination Reactions -- Complement Fixation -- Neutralization Assays -- Immunofluorescence Assays -- Enzyme Immunoassays -- Chemiluminescence Immunoassays -- Western Blotting and Immunoblotting.
Rapid Immunoassays -- Automated Technologies -- SUMMARY -- REFERENCES -- 8 Prevention of Health Care-Associated Infections -- HEALTH CARE-ASSOCIATED (OR NOSOCOMIAL) INFECTION -- Definition -- Infection Rates, Infection Sites, and Predominant Pathogens -- Morbidity, Mortality, and Cost -- HOSPITAL INFECTION PREVENTION PROGRAM -- Structure, Personnel, and Responsibility -- Infection Prevention Committee -- HAI Surveillance -- Process Surveillance -- Antimicrobial Stewardship -- ROLE OF THE CML IN INFECTION PREVENTION -- Specimen Collection and Transport -- Accurate Identification and Susceptibility Testing of HAI Pathogens -- Laboratory Information Systems -- Rapid Diagnostic Testing for Infection Prevention and Antimicrobial Stewardship -- Reporting Laboratory Data to the Infection Prevention Program -- Outbreak Recognition and Investigation: Epidemic versus Endemic Infections -- Molecular Typing to Support Infection Control Activities -- Organism Banking and Storage -- Surveillance Cultures from Patients, Hospital Personnel, and the Environment -- Emerging Issues and Trends in Health Care Infection Prevention -- CONCLUSION -- REFERENCES -- 9 Investigation of Disease Outbreaks -- INTRODUCTION/DEFINITIONS -- DISEASE SURVEILLANCE -- OUTBREAK DETECTION -- EPIDEMIOLOGICAL APPROACHES TO AN OUTBREAK INVESTIGATION -- ROLE OF THE LABORATORY IN OUTBREAKS -- Routine -- Current and Novel Lab Detection Methods (Illustrative Examples) -- SELECT EXAMPLES OF OUTBREAK SCENARIOS: HEALTH CARE AND FACILITIES, CONTAMINATED PRODUCTS, AND FOODBORNE ILLNESSES -- RESOURCES TO STAY INFORMED AND CONNECTED IN GLOBAL OUTBREAKS -- CONCLUSIONS -- REFERENCES -- 10 Molecular Epidemiology -- SUBTYPING METHODS -- Subtyping Method Characteristics -- Non-Target-Specific Methods -- Amplified Fragment Length Polymorphism -- Target-Specific Methods -- Whole-Genome SNP Typing.
WGS, MLST, and Binary Typing -- k-mer Analysis -- Mass Spectrometry -- APPLICATIONS -- Molecular Surveillance -- Forensic Microbiology -- SUBTYPING METHOD SELECTION, VALIDATION, AND DATA INTERPRETATION -- Method Selection -- Method Validation -- Data Interpretation -- LIBRARIES FOR MOLECULAR EPIDEMIOLOGY -- Strain Catalogues -- Surveillance Databases -- CONCLUSIONS AND FUTURE TRENDS -- REFERENCES -- 11 Procedures for the Storage of Microorganisms -- OVERVIEW OF PRESERVATION METHODS -- Short-Term Preservation Methods -- Long-Term Preservation Methods -- DISASTER PREPAREDNESS -- FUTURE DIRECTIONS -- REFERENCES -- 12 Prevention of Laboratory-Acquired Infections -- EPIDEMIOLOGY -- LABORATORY BIOSAFETY PROGRAM -- LABORATORY SAFETY AND RISK MANAGEMENT -- LABORATORY SAFETY AND PERSONAL ATTRIBUTES -- RISK-BASED CLASSIFICATION OF MICROORGANISMS -- BIOSAFETY AND CLINICAL LABORATORY DESIGN -- SAFETY EQUIPMENT AND THE CLINICAL LABORATORY -- Splashguards -- Biosafety Cabinets -- Chemical Fume Protection -- Installation of Biological Safety Cabinets and Chemical Fume Hoods -- Centrifuges -- Sharps Protection -- Medical Waste as an Infection Hazard -- SAFETY AS A QUALITY MANAGEMENT INITIATIVE -- Safety Preparedness and the Material Safety Data Sheet -- Hand Washing and the Use of Personal Protective Equipment -- Gloves -- Respirator Masks -- Immunization -- Notable Laboratory-Acquired Infections -- Safety and Point of Care -- Safety Considerations Associated with MALDI-TOF Mass Spectrometry -- Transportation of Samples -- Postexposure Management for Accidents Involving Infectious Agents -- REFERENCES -- 13 Decontamination, Disinfection, and Sterilization -- PRINCIPLES, TERMINOLOGY, AND BACKGROUND -- ANTISEPTICS -- Common Antiseptic Compounds -- Hygienic Hand Washing and Hand Disinfection -- Surgical Hand Washing (Scrub) or Surgical Hand Disinfection (Rub-In).
Bibliography Note Includes bibliographical references and index.
Summary "The Gold Standard for medical microbiology, diagnostic microbiology, clinical microbiology, infectious diseases due to bacteria, viruses, fungi, parasites; laboratory and diagnostic techniques, sampling and testing, new diagnostic techniques and tools, molecular biology; antibiotics/ antivirals/ antifungals, drug resistance; individual organisms (bacteria, viruses, fungi, parasites)"--Publisher's description.
ISBN 9781555817381
1555817386
1555817378
9781555817374
OCLC # 994501573