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Title Antiviral methods and protocols / edited by Derek Kinchington and Raymond F. Schinazi.
Imprint Totowa, N.J. : Humana Press, [2000]
©2000

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Series Methods in molecular medicine ; 24.
Methods in molecular medicine ; 24.
Subject Antiviral agents -- Laboratory manuals.
Hepatitis, Viral, Human -- drug therapy.
Antiviral Agents -- therapeutic use.
DNA Virus Infections -- drug therapy.
RNA Virus Infections -- drug therapy.
Alt Name Kinchington, Derek.
Schinazi, Raymond F.
Description 1 online resource (xiv, 404 pages) : illustrations.
Bibliography Note Includes bibliographical references and index.
Contents Changing methods for discovering antiviral drugs / P.S. Jones-Laboratory safety considerations / P.M. Feorino, J.D. Williamson and R.F. Schinazi-Routine quality control testing of cell cultures : Detection of mycoplasma / A. Stacey and G. Stacey-Hep ad38 assay : A high-throughput, cell-based screen for the evaluation of compounds against hepatitis b virus / R.W. King and S.K. Ladner-Hepatitis b virus cell culture assays for antiviral activity / K. Schmidt and B. Korba-A rapid microtiter assay for duck hepatitis virus reverse transcriptase / M.J. Otto and R.A. Whitaker-Assays for measuring hepadnaviral supercoiled DNA / S.A. Locamini and G.M. Civitico-Assays for hepatitis b virus DNA-and rna-dependent DNA polymerase activities / T. Shaw and S.A. Locarnini-Development of a hepatitis c virus rna helicase high throughput assay / A.D. Kwong and C. Risano-Determination of viral infectivity : Hsv, vzv, and cmv / D.R. Harper-Antiviral screening assays for human herpesviruses 6 and 7 / J.B. Black and P.E. Pellett-Strategies for evaluation of antiviral agents against epstein-barr virus in culture / J.C. Lin-Monitoring drug resistance for herpesviruses / G. Andrei [and others]-Herpesvirus protease assays / P. Ertl, L. Russell and J. Angier-Testing compounds for antiviral activity in cell cultures infected with hiv / D. Devine, N. Mathews and D. Kinchington-Evaluation of compounds that prevent reactivation of hiv-1 in om-10.1 cells / S.T. Butera-Assessment of activity of topical virucidal agents / O.C. T-Assays that measure selective killing of virus-infected cells / S. Randall-Evaluating clinical isolates for their phenotypic and genotypic resistance against anti-hiv drugs / A.M. Vandamme [and others]-Line probe assay for detecting mutations in hiv-1 reverse transcriptase / L. Stuyver [and others]-Rt-pcr for amplification of specific fragments of hiv-1 genome / M.P. de Bethune and K. Hertogs-Evaluation of compounds against recombinant hiv reverse transcriptase / J. Balzarini-Ultrasensitive detection of reverse transcriptase activity by the amp-rt assay : Applications to the measurement of virus loads and phenotypic drug resistance / J. Gerardo, L. Garcia and W. Heneine-Reverse transcriptase assay based on product enhancement for assessing the drug susceptibility of retroviruses / J. Boni and J. Schupbach-Identifying and characterizing hiv protease inhibitors / E.S. Furfine-Retroviral integrase : A novel target in antiviral drug development and basic in vitro assays with the purified enzyme / A. Mazumder [and others]-Papillomavirus DNA replication / M.C. Ferran and A.A. McBride-Quantitative immunodetection of viral oncoprotein levels in human papillomavirus-transformed human cells / J.A. Johnson and J.D. Gangemi-Biochemical methods for the characterization of influenza viruses with reduced sensitivity to 4-guanidino-neu5ac2en / R.C. Bethell [and others]-Virological methods for the generation and characterization of influenza viruses with reduced sensitivity to 4-guanidino-neu5ac2en / J.L. McKimm-Breschkin [and others].
Access Use copy Restrictions unspecified star
Reproduction Electronic reproduction. [S.l.] : HathiTrust Digital Library, 2010.
System Details Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212
Summary In Antiviral Methods and Protocols, experienced scientists describe-in an easily-followed format-their state-of-the-art techniques for evaluating antiviral compounds. The assays described include systems for investigating drugs used against herpesviruses, hepatitis viruses, human immunodeficiency viruses, human papillomaviruses, and influenza viruses. These well-tested methods range from cellular assays to some of the most up-to-date molecular approaches for identifying compounds that are active against viral enzymes and the development of viral resistance against drugs currently in use. The detailed protocols also discuss possible pitfalls and ways to overcome them. Timely and comprehensive, Antiviral Methods and Protocols offers today's researchers in academia, clinical departments, and the pharmaceutical industry the powerful, reproducible, and novel methods needed to evaluate compounds effective against both acute and chronic infections.
Note Print version record.
ISBN 0585235686 (NetLibrary e-ISBN)
9780585235684 (NetLibrary e-ISBN)
9781592592456 (Springer e-ISBN)
1592592457 (Springer e-ISBN)
0896035611 (acid-free paper)
9780896035614 (acid-free paper)
OCLC # 45729995
Additional Format Print version: Antiviral methods and protocols. Totowa, N.J. : Humana Press, ©2000 0896035611 (DLC) 99025099 (OCoLC)41096164.
Table of Contents
 Preface 
 List of Contributors 
1Changing Methods for Discovering Antiviral Drugs / Philip S. Jones1
2Laboratory Safety Considerations / Paul M. Feorino, John D. Williamson, Raymond F. Schinazi13
3Routine Quality Control Testing of Cell Cultures: Detection of Mycoplasma / Alison Stacey, Glyn Stacey27
4Hep AD38 Assay: A High-Throughput, Cell-Based Screen for the Evaluation of Compounds Against Hepatitis B Virus / Robert W. King, Stephanie K. Ladner43
5Hepatitis B Virus Cell Culture Assays for Antiviral Activity / Karen Schmidt, Brent Korba51
6A Rapid Microtiter Assay for Duck Hepatitis Virus Reverse Transcriptase / Michael J. Otto, Richard A. Whitaker69
7Assays for Measuring Hepadnaviral Supercoiled DNA / Stephen A. Locarnini, Gilda M. Civitico77
8Assays for Hepatitis B Virus DNA- and RNA-Dependent DNA Polymerase Activities / Timothy Shaw, Stephen A. Locarnini87
9Development of a Hepatitis C Virus RNA Helicase High Throughput Assay / Ann D. Kwong, Christine Risano97
10Determination of Viral Infectivity: HSV, VZV, and CMV / David R. Harper119
11Antiviral Screening Assays for Human Herpesviruses 6 and 7 / Jodi B. Black, Philip E. Pellett129
12Strategies for Evaluation of Antiviral Agents Against Epstein-Barr Virus in Culture / Jung-Chung Lin139
13Monitoring Drug Resistance for Herpesviruses [et al.] / Graciela Andrei, Pierre Fiten, Erik De Clercq151
14Herpesvirus Protease Assays / Peter Ertl, Linda Russell, Jane Angier171
15Testing Compounds for Antiviral Activity in Cell Cultures Infected with HIV / Donna Devine, Naomi Mathews, Derek Kinchington185
16Evaluation of Compounds That Prevent Reactivation of HIV-1 in OM-10.1 Cells / Salvatore T. Butera201
17Assessment of Activity of Topical Virucidal Agents / Timothy O'Connor207
18Assays That Measure Selective Killing of Virus-Infected Cells / Sharon Randall213
19Evaluating Clinical Isolates for Their Phenotypic and Genotypic Resistance Against Anti-HIV Drugs [et al.] / Anne-Mieke Vandamme, Myriam Witvrouw, Christophe Pannecouque223
20Line Probe Assay for Detecting Mutations in HIV-1 Reverse Transcriptase [et al.] / Lieven Stuyver, Ann Wyseur, Gonda Verpooten259
21RT-PCR for Amplification of Specific Fragments of HIV-1 Genome / Marie-Pierre de Bethune, Kurt Hertogs269
22Evaluation of Compounds Against Recombinant HIV Reverse Transcriptase / Jan Balzarini283
23Ultrasensitive Detection of Reverse Transcriptase Activity by the Amp-RT Assay: Applications to the Measurement of Virus Loads and Phenotypic Drug Resistance / J. Gerardo Garcia Lerma, Walid Heneine291
24Reverse Transcriptase Assay Based on Product Enhancement for Assessing the Drug Susceptibility of Retroviruses / Jurg Boni, Jorg Schupbach301
25Identifying and Characterizing HIV Protease Inhibitors / Eric S. Furfine313
26Retroviral Integrase: A Novel Target in Antiviral Drug Development and Basic In Vitro Assays with the Purified Enzyme [et al.] / Abhijit Mazumder, Nouri Neamati, Sanjay Sunder327
27Papillomavirus DNA Replication / Maureen C. Ferran, Alison A. McBride341
28Quantitative Immunodetection of Viral Oncoprotein Levels in Human Papillomavirus-Transformed Human Cells / Jeffery A. Johnson, J. David Gangemi361
29Biochemical Methods for the Characterization of Influenza Viruses with Reduced Sensitivity to 4-Guanidino-Neu5Ac2en [et al.] / Richard C. Bethell, Graham J. Hart, Tony J. Blick367
30Virological Methods for the Generation and Characterization of Influenza Viruses with Reduced Sensitivity to 4-Guanidino-Neu5A2en [et al.] / Jennifer L. McKimm-Breschkin, Anjali Sahasrabudhe, Tony J. Blick375
 Index383