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Author Denomme, Gregory A.
Title Molecular protocols in transfusion medicine / Gregory A. Denomme, Maria Rios, Marion E. Reid.
Imprint San Diego, CA : Academic Press, 2000.

Author Denomme, Gregory A.
Subject Blood groups -- Handbooks, manuals, etc.
Genetic polymorphisms -- Handbooks, manuals, etc.
Blood -- Transfusion -- Handbooks, manuals, etc.
Blood Grouping and Crossmatching.
Blood Transfusion.
Polymorphism, Genetic.
Alt Name Rios, Maria.
Reid, Marion E.
Description 1 online resource (x, 186 pages) : illustrations
polychrome rdacc
Summary This essential methods manual for immunohematologists (or hematologists and immunohematologists) provides information on genes that encode antigens on red blood cells, platelets and neutrophils. The book begins by covering general concepts in molecular biology and specific protocols such as DNA preparation, PCR-RFLP and allele-specific PCR. Information on the erythrocyte, platelet and neutrophil antigen systems and the molecular basis of polymorphisms are presented clearly in a gene facts sheet format. Database accession numbers and useful adjuncts such as Request forms, worksheets for PCR/enzyme digests also serve to benefit the user. The information is clearly presented and easily accessible and is complemented by the excellent diagrams and tabular material. This book is invaluable for both new and experienced researchers in the field and other related disciplines. Key Features * Essential for hematologists and those involoved in tissue typing and the study of human genetic polymorphisms * Presents clearly and concisely the information on a particular variant and the technique used to detect it * Organized by antigen and provides sequences of polymorphisms and primers * Details the general concepts and critical information on genes, their products, and sources of relevant nucleic acids * Includes protocols that allow investigators to set up assays with minimal effort (protocols include primers, reagents, reaction conditions, sizes of amplified products, restriction fragment digests, and the relevant safety information) * Provides information that helps interpret results in clinical settings * Contains additional sources of information (e.g., key references, web site addresses, glossary, Database accession numbers, request forms, and worksheets for PCR/enzyme digests).
Bibliography Note Includes bibliographical references and index.
Contents Front Cover; Molecular Protocols in Transfusion Medicine; Copyright Page; Contents; Preface; List of Abbreviations; Part 1: Introduction; Part 2: General Considerations; Part 3: Protocols; Part 4: Red Cell Blood Groups; Part 5: Platelet Blood Groups; Part 6: Neutrophil Blood Groups; Part 7: Appendices; Index; Useful Tables.
Note Print version record.
ISBN 9780122093708
9781435608092 (electronic bk.)
1435608097 (electronic bk.)
OCLC # 162129287
Additional Format Print version: Denomme, Gregory A. Molecular protocols in transfusion medicine. San Diego, CA : Academic Press, 2000 0122093704 9780122093708 (DLC) 99068336 (OCoLC)44184819.

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