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LEADER 00000cam  2200733Ia 4500 
001    162129287 
003    OCoLC 
005    20190816064556.4 
006    m     o  d         
007    cr cn||||||||| 
008    070802s2000    caua    obf   001 0 eng d 
015    GBB739584|2bnb 
019    180757165|a182732302|a647695710|a823828618|a823898354
020    9780122093708 
020    0122093704 
020    9781435608092|q(electronic bk.) 
020    1435608097|q(electronic bk.) 
020    0080536905 
020    9780080536903 
020    128103276X 
020    9781281032768 
035    (OCoLC)162129287|z(OCoLC)180757165|z(OCoLC)182732302
037    88709:88709|bElsevier Science & Technology|nhttp:// 
040    OPELS|beng|epn|erda|cOPELS|dOCLCQ|dN$T|dYDXCP|dIDEBK|dE7B
049    MAIN 
050  4 QP98|b.D46 2000eb 
060  4 QY 415|bD413m 2000 
060  4 2000 H-750 
072  7 SCI|x036000|2bisacsh 
072  7 MED|x075000|2bisacsh 
072  7 MJCM|2bicssc 
082 04 612.1/1825|222 
100 1  Denomme, Gregory A.|0
245 10 Molecular protocols in transfusion medicine /|cGregory A. 
       Denomme, Maria Rios, Marion E. Reid. 
264  1 San Diego, CA :|bAcademic Press,|c2000. 
300    1 online resource (x, 186 pages) :|billustrations 
336    text|btxt|2rdacontent 
337    computer|bc|2rdamedia 
338    online resource|bcr|2rdacarrier 
340    |gpolychrome|2rdacc|0
347    text file|2rdaft|0
504    Includes bibliographical references and index. 
505 0  Front Cover; Molecular Protocols in Transfusion Medicine; 
       Copyright Page; Contents; Preface; List of Abbreviations; 
       Part 1: Introduction; Part 2: General Considerations; Part
       3: Protocols; Part 4: Red Cell Blood Groups; Part 5: 
       Platelet Blood Groups; Part 6: Neutrophil Blood Groups; 
       Part 7: Appendices; Index; Useful Tables. 
520    This essential methods manual for immunohematologists (or 
       hematologists and immunohematologists) provides 
       information on genes that encode antigens on red blood 
       cells, platelets and neutrophils. The book begins by 
       covering general concepts in molecular biology and 
       specific protocols such as DNA preparation, PCR-RFLP and 
       allele-specific PCR. Information on the erythrocyte, 
       platelet and neutrophil antigen systems and the molecular 
       basis of polymorphisms are presented clearly in a gene 
       facts sheet format. Database accession numbers and useful 
       adjuncts such as Request forms, worksheets for PCR/enzyme 
       digests also serve to benefit the user. The information is
       clearly presented and easily accessible and is 
       complemented by the excellent diagrams and tabular 
       material. This book is invaluable for both new and 
       experienced researchers in the field and other related 
       disciplines. Key Features * Essential for hematologists 
       and those involoved in tissue typing and the study of 
       human genetic polymorphisms * Presents clearly and 
       concisely the information on a particular variant and the 
       technique used to detect it * Organized by antigen and 
       provides sequences of polymorphisms and primers * Details 
       the general concepts and critical information on genes, 
       their products, and sources of relevant nucleic acids * 
       Includes protocols that allow investigators to set up 
       assays with minimal effort (protocols include primers, 
       reagents, reaction conditions, sizes of amplified products,
       restriction fragment digests, and the relevant safety 
       information) * Provides information that helps interpret 
       results in clinical settings * Contains additional sources
       of information (e.g., key references, web site addresses, 
       glossary, Database accession numbers, request forms, and 
       worksheets for PCR/enzyme digests). 
588 0  Print version record. 
650  0 Blood groups|0
       sh85014985|vHandbooks, manuals, etc.|0
650  0 Genetic polymorphisms|0
       subjects/sh85053865|vHandbooks, manuals, etc.|0http:// 
650  0 Blood|xTransfusion|0
       /sh85014949|vHandbooks, manuals, etc.|0
650 12 Blood Grouping and Crossmatching.|0
650 22 Blood Transfusion.|0 
650 22 Polymorphism, Genetic.|0
655  2 Laboratory Manual.|0 
655  4 Electronic books. 
655  7 Handbooks and manuals.|2fast|0
655  7 Handbooks and manuals.|2lcgft|0
700 1  Rios, Maria.|0
700 1  Reid, Marion E.|0
776 08 |iPrint version:|aDenomme, Gregory A.|tMolecular protocols
       in transfusion medicine.|dSan Diego, CA : Academic Press, 
       2000|z0122093704|z9780122093708|w(DLC)   99068336
956 40 |u
       db=nlebk&AN=141468|zView online 
990    eBooks on EBSCOhost|bEBSCO eBook Subscription Academic 
       Collection - North America|c2019-08-16|yAdded to 
       collection netlibrary.academicna|5OHN 

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