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Title Molecular diagnostics : for the clinical laboratorian / edited by William B. Coleman and Gregory J. Tsongalis ; foreword by Lawrence M. Silverman. LMS PhD;Char. U of Virginia.
Imprint New York : Humana Press/Springer Science+Business Media, 2010.
2006
Edition Second edition, (corrected at fourth printing 2010).

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LOCATION CALL # STATUS MESSAGE
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Subject Molecular diagnosis.
Pathology, Molecular.
Medical Laboratory Science.
Chemistry, Clinical -- methods.
Genetic Techniques.
Clinical Laboratory Techniques.
Molecular Biology -- methods.
Alt Name Coleman, William B.,
Tsongalis, Gregory J.,
Description 1 online resource (xvi, 567 pages) : illustrations
monochrome rdacc
Edition Second edition, (corrected at fourth printing 2010).
Bibliography Note Includes bibliographical references and index.
Summary "Taking advantage of the many major advances that have occurred since their groundbreaking first edition was published, William B. Coleman and Gregory J. Tsongalis have updated and expanded their highly praised tutorial guide to molecular diagnostic techniques to include not only improved traditional methods, but also totally new molecular technologies, some not yet in routine use. The authors offer cutting-edge molecular diagnostics for genetic disease, human cancers, infectious diseases, and identity testing, as well as new insights into the question of quality assurance in the molecular diagnostics laboratory. Additional chapters address other technologies found in the clinical laboratory that are complementary to molecular diagnostic methodologies, and also discuss genetic counseling and the ethical and social issues involved with nucleic acid testing. ... the essential textbook of choice for anyone working in molecular diagnostics and who wants to remain current with this rapidly changing field."--Provided by publisher.
Contents pt. I. Introduction. An historical perspective on the clinical diagnostic laboratory / Robert E. Moore -- pt. II. Basic molecular biology. An overview of nucleic acid chemistry, structure, and function: the foundations of molecular biology / William B. Coleman -- Extraction of nucleic acids / Paul N. Bogner and Anthony A. Killeen -- Nucleic acid blotting techniques: theory and practice / Terry Amiss and Sharon Collins Presnell -- The polymerase chain reaction / William B. Coleman and Gregory J. Tsongalis -- Bioinformatics: computer-based approaches to genetic analysis / Sharon L. Ricketts -- pt. III. Molecular diagnostic technologies. PCR-based methods for mutation detection / Ian M. Frayling, Emma Monk, and Rachel Butler -- Alternative methods for amplified nucleic acid testing / Deborah A. Payne and Laurie E. Sower -- Electrophoretic methods for mutation detection and DNA sequencing / W. Edward Highsmith, Jr. -- Single-nucleotide polymorphisms: testing DNA variation for disease association / Ulrich Broeckel and Martin J. Hessner -- Microarray approaches to gene expression analysis / David Neil Hayes and Matthew Meyerson -- Methods for analysis of DNA methylation / Alexander Dobrovic -- pt. IV. Other clinical diagnostic technologies / Flow Cytometry / Joseph A. DiGiuseppe.
Medical cytogenetics / Martha B. Kaegle -- Fluorescence In situ hybridization: a major milestone in luminous cytogenetics / Suneel D. Mundle and Robert J. Koska -- Immunohistochemistry / John Hunt [and others] -- Lser capture microdissection / C. Robert Bagnell, Jr. -- pt. V. Quality assurance in the molecular diagnostics laboratory. Framework for quality assurance in molecular diagnostics / Marlene Sabbath-Solitare, Selwyn J. Baptist, and Teresita Cuyegkeng Redondo -- Verification of molecular assays / Brent L. Seaton -- Standards and standardization of molecular diagnostics / John P. Jakupciak and Catherine D. O'Connell -- Laboratory-developed tests in molecular diagnostics / Andrea Ferreira-Gonzalez and Carleton T. Garrett -- pt. VI. Applications of molecular diagnostics for genetic diseases. An overview of molecular genetics / Elaine Weidenhammer and Gregory J. Tsongalis -- Genetic basis of neurologic neuromuscular diseases / Narasimhan Nagan [and others] -- Molecular mechanisms of endocrine disorders / Amy Potter and John A. Phillips III -- Molecular pathogenesis of cardiovascular disease / Alan H.B. Wu -- Molecular diagnostics in coagulation / Enrique Ballesteros -- Cystic fibrosis / Eugene H. Lewis III [and others].
Prenatal genotyping for identification of identification of fetuses at risk for immune cytopenic disorders / Martin J. Hessner and Brian R. Curtis -- Personalized medicine / Karen McCullough -- pt. VII. Applications of molecular diagnostics for human cancers. Molecular pathogenesis of human cancer / William B. Coleman and Gregory J. Tsongalis -- Application of molecular diagnostics to hereditary nonpolyposis colorectal cancer / Ian M. Frayling [and others] -- Molecular genetic applications to the diagnosis of lymphoma / William N. Rezuke, Jr. and Gregory J. Tsongalis -- Molecular genetic abnormalities in acute and chronic leukemias / Pei Lin and L. Jeffrey Medeiros -- pt. VIII. Applications of molecular diagnostics for infectious diseases. Molecular testing for Chlamydia trachomatis and Neisseria gonorrhoeae / Alexandra Valsamakis -- Human Papillomavirus / Theresa M. Voytek and Gregory J. Tsongalis -- Molecular diagnosis for HIV-1 / Angela M. Caliendo and Charles E. Hill -- Hepatitis C / D. Robert Dufour.
Cytomegalovirus / Angela M. Caliendo and Nell S. Lurain -- pt. IX. Applications of molecular diagnostics for identity-based testing. HLA typing using molecular methods / John L. Schmitz -- Molecular analysis for forensic casework and parentage testing / Tracey Dawson Cruz -- Molecular assessment of bone marrow transplant engraftment / Jessica K. Booker -- Use of DNA-based identity testing for specimen identification / Andrew Ricci Jr. and Gregory J. Tsongalis -- pt. X. Issues for the clinical molecular pathology laboratory. Genetic counseling considerations in molecular diagnosis / Myra I. Roche -- Ethical, social, and legal issues related to molecular genetic testing / Jennifer L. Herbst and Jon F. Merz.
Note Online resource; title from e-book title screen (EBL platform, viewed February 6, 2017).
Print version record.
ISBN 9781592599288 (electronic bk.)
1592599281 (electronic bk.)
9781592599257 (eISBN)
1592599257 (eISBN)
9781588293565 (alk. paper)
1588293564 (alk. paper)
OCLC # 655857203
Additional Format Print version: Molecular diagnostics. 2nd ed. Humana Press : Totowa, N.J., 2006 1588293564 9781588293565 (DLC) 2004023555 (OCoLC)56682830


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