| Author |
Mächtle, Walter.
|
| Series |
Springer laboratory |
|
Springer laboratory.
|
| Subject |
Ultracentrifugation.
|
|
Polymers -- Analysis.
|
|
Nanoparticles -- Analysis.
|
|
Ultracentrifugation -- methods.
|
|
Polymers -- analysis.
|
|
Nanostructures -- analysis.
|
| Alt Name |
Börger, L.
|
| Description |
1 online resource (xiii, 237 pages) : illustrations. |
|
polychrome rdacc |
| Bibliography Note |
Includes bibliographical references and index. |
| Summary |
Annotation Analytical ultracentifugation (AUe is a powerful method for the characterization of polymers, biopolymers, polyelectrolytes, nanoparticles, dispersions, and other colloidal systems. The method is able to determine the molar mass, the particle size, the particle density and interaction parameters like virial coefficients and association constants. Because AUC is also a fractionation method, the determination of the molar mass distribution, the particle size distribution, and the particle density distribution is possible. A special technique, the density gradient method, allows fractionating heterogeneous samples according to their chemical nature that means being able to detect chemical heterogeneity. The book is divided into chapters concerning instrumentation, sedimentation velocity runs, density gradient runs, application examples and future developments. In particular, the detailed application chapter demonstrates the versatility and power of AUC by means of many interesting and important industrial examples. Thus the book concentrates on practical aspects rather than details of centrifugation theory. Both authors have many years of experience in an industrial AUC research laboratory of a world leading chemical company. |
| Contents |
Cover -- Table of Contents -- 1 INTRODUCTION -- 1.1 Historic Examples of Ultracentrifugation -- 1.1.1 Investigations on Gold Colloids in 1924 -- 1.1.2 Investigations on the Structure of DNA in 1957 -- 1.2 Basic Theory ofUltracentrifugation -- 1.2.1 Svedberg's Simplified Theory -- 1.2.2 Derivation of Lamm's Equation -- 1.3 Basic Experiment Types of Ultracentrifugation -- 1.3.1 Sedimentation Velocity Experiment -- 1.3.2 Synthetic Boundary Experiment -- 1.3.3 Sedimentation Equilibrium -- 1.3.4 Density Gradient -- 1.3.5 Approach-to-Equilibrium(or Archibald)Method -- 1.4 Closing Remarks -- References -- 2 ANALYTICAL ULTRACENTRIFUGATION, INSTRUMENTATION -- 2.1 Ultracentrifuges -- 2.1.1 The Beckman-CoulterOptima XL-A/I -- 2.1.2 User-Made Centrifuges -- 2.2 Rotors -- 2.3 Measuring Cells -- 2.4 Detectors -- 2.4.1 AbsorptionOptics -- 2.4.2 Interference Optics -- 2.4.3 Schlieren Optics -- 2.4.4 OtherDetectors -- 2.5 Multiplexer -- 2.6 AuxiliaryMeasurements -- 2.6.1 Measurement of the Solvent Density and the Partial Specific Volume -- 2.6.2 Measurement of the Refractive Index and the Specific Refractive Index Increment -- References -- 3 SEDIMENTATION VELOCITY -- 3.1 Introduction -- 3.2 Basic Example of Sedimentation Velocity -- 3.2.1 Determination of s -- 3.2.2 Standard Conditions for s Estimation -- 3.2.3 Radial Dilution and Thickening -- 3.2.4 Concentration Dependence -- 3.3 Advanced Theory of Sedimentation Velocity Runs -- 3.3.1 Johnston-Ogston Effect -- 3.3.2 Self-Sharpening of Boundaries -- 3.3.3 Pressure Dependence -- 3.3.4 Speed Dependence -- 3.3.5 Charge Effects -- 3.3.6 Separation of Sedimentation and Diffusion -- 3.3.7 Test of Homogeneity -- 3.4 Sedimentation Velocity Runs of Macromolecules to Measure Average M and MMD -- 3.4.1 Evaluation of the Average Molar Mass M by Sedimentation Velocity Runs via Scaling Laws -- 3.4.2 Evaluation of Molar Mass Distributions (MMD) by Sedimentation Velocity Runs via Scaling Laws -- 3.5 Sedimentation Velocity Runs on Particles to Measure Average d[sub(p)] and PSD -- 3.5.1 Particle Size Distribution via AUC Turbidity Detector and Mie Theory -- 3.5.2 Coupling Technique to Measure very Broad PSD -- 3.5.3 H[sub(2)]O-D[sub(2)]O Density Variation Method to Measure Particle Densities via Sedimentation Velocity Runs -- 3.5.4 PSD Measurement of very Small Platinum Clusters Using UVOptics -- 3.6 Synthetic Boundary Experiments -- 3.6.1 Synthetic Boundary Crystallization Ultracentrifugation -- References -- 4 DENSITY GRADIENTS -- 4.1 Introduction -- 4.2 Static Density Gradients -- 4.2.1 Theory of StaticDensity Gradients -- 4.2.2 Gradient Materials -- 4.2.3 Experimental Procedure -- 4.2.4 Examples -- 4.3 Dynamic Density Gradients -- 4.4 Other Types of Den. |
| Note |
Print version record. |
| ISBN |
9783540262183 |
|
3540262180 |
|
3540234322 (hd. bd.) |
|
9783540234326 (hd. bd.) |
|
6610625514 |
|
9786610625512 |
| ISBN/ISSN |
9783540234326 |
| OCLC # |
262691788 |
| Link |
Springer e-books |
| Additional Format |
Print version: Machtle, Walter. Analytical ultracentrifugation of polymers and nanoparticles. Berlin ; New York : Springer, 2006 3540234322 9783540234326 (DLC) 2005933893 (OCoLC)64571899 |
|
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