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Title Practical manual of in vitro fertilization : advanced methods and novel devices / Zsolt Peter Nagy, Alex C. Varghese, Ashok Agarwal, editors.
Imprint New York : Springer, 2012.

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Subject Fertilization in vitro, Human -- Handbooks, manuals, etc.
Fertilization in Vitro -- methods.
Clinical Laboratory Techniques -- standards.
Alt Name Nagy, Zsolt Peter.
Varghese, Alex C.
Agarwal, Ashok (Physician)
Description 1 online resource (xxiii, 703 pages)
polychrome rdacc
Bibliography Note Includes bibliographical references and index.
Contents Part 1. Setting Up and Running an IVF Laboratory / Building the Laboratory / Dean E. Morbeck and Marlena Duke -- Air Quality Management / Johan Guns, Ronny Janssens and Martine Vercammen -- Organizational Aspects of the Laboratory in a Tertiary Care ART Center / Nancy L. Bossert and Christopher De Jonge -- Quality Control Management / William R. Boone and H. Lee Higdon -- Daily, Weekly, and Regular Preparations for the IVF Laboratory / Michael A. Britt and Klaus E. Wiemer -- Risk and Safety in the IVF Clinic / Peter Sjoblom and Julius Hreinsson -- Journey of Human Gametes In Vitro: 1978-2010 / Zeev Shoham.
Part 2. IVF Laboratory Equipment and Culture Systems -- Essential Instruments and Disposable Supplies for an IVF Laboratory / Leslie Weikert, Christa Fralick and Klaus E. Wiemer -- Co2 and Low-O2 Incubators / Marius Meintjes -- IVF Workstations / Nicolas Prados and Alex C. Varghese -- Culture Media in IVF: Decisions for the Laboratory / Jason E. Swain and Thomas B. Pool.
Part 3. In Vitro Fertilization -- Oocyte Denuding / Roberta Maggiulli, Filippo Ubaldi and Laura Rienzi -- Assessment of Oocyte Quality / Basak Balaban, Turgay Barut and Bulent Urman -- Polarization Microscopy / Markus Montag, Maria Koster and Hans van der Ven -- Cumulus Cell Gene Expression in Assessment of Oocyte Quality / Dagan Wells.
Part 4. Embryo Culture Methods -- Short Culture: Day 1/Day 2/Day 3 Embryo Culture / Patrick Quinn -- Extended Culture in IVF / David K. Gardner and Michelle Lane -- In Vitro Maturation of Human Oocytes / Baris Ata, Jack Huang and Ri-Cheng Chian -- In Vivo Embryo Culture Device / Claude Ranoux -- Microfluidics for Gamete Manipulation and Embryo Culture / Gary D. Smith, Charles Bormann and Shuichi Takayama.
Part 5. Sperm Processing and Selection -- Sperm Assessment: Traditional Approaches and Their Indicative Value / Margot Flint, Fanuel Lampiao, Ashok Agarwal and Stefan S. du Plessis -- Sperm Assessment: Novel Approaches and Their Indicative Value / De Yi Liu, Harold Bourne, Claire Garrett, Gary N. Clarke and Shlomi Barak, et al. -- Sperm Processing for IVF / Ralf Henkel -- PESA/TESA/TESE Sperm Processing / Sandro C. Esteves and Sidney Verza -- Processing Sperm Samples in HIV-Positive Patients / Thamara Viloria, Marcos Meseguer, Antonio Pellicer, Jose Remohi and Nicolas Garrido -- Intracytoplasmic Morphologically Selected Sperm Injection / P. Vanderzwalmen, Magnus Bach, Batsuren Baramsai, A. Neyer and Delf Schwerda, et al. -- Sperm Testing and ICSI Selection by Hyaluronic Acid Binding: The Hyaluronic Acid-Coated Glass Slide and Petri Dish in the Andrology and IVF Laboratories / Gabor Huszar -- Electrophoretic Sperm Separation / Steven Fleming and John Aitken -- Magnetic-Activated Cell Sorting of Human Spermatozoa / Enver Kerem Dirican -- Polscope-Based Sperm Selection / Luca Gianaroli, Cristina Magli, Andor Crippa, Giorgio Cavallini and Eleonora Borghi, et al.
Part 6. Insemination Procedures -- Intrauterine Insemination / Gautam N. Allahbadia and Rubina Merchant -- Conventional IVF Insemination / Liesl Nel-Themaat, Thomas Elliott, Ching-Chien Chang, Graham Wright and Zsolt Peter Nagy -- Intracytoplasmic Sperm Injection / Gianpiero D. Palermo, Queenie V. Neri, Devin Monahan, Takumi Takeuchi and Peter N. Schlegel, et al. -- Mechanism of Human Oocyte Activation During ICSI and Methodology for Overcoming Low or Failed Fertilization / Dmitri Dozortsev and Mohammad Hossein Nasr-Esfahani.
Part 7. Micromanipulators and Micromanipulation -- Hydraulic Manipulators for ICSI / Hubert Joris -- Research Instruments Micromanipulators / Steven Fleming and Catherine Pretty -- Eppendorf Micromanipulator: Setup and Operation of Electronic Micromanipulators / Ehab Abu-Marar and Safa Al-Hasani -- The Leica Microsystems' IMSI System / Christiane Wittemer, Bruno Laborde, Frederic Ribay and Stephane Viville -- Automated Robotic Intracytoplasmic Sperm Injection / Zhe Lu, Xinyu Liu, Xuping Zhang, Clement Leung and Navid Esfandiari, et al. -- Oocyte Treatment and Preparation for Microinjection / Thomas Ebner -- Livestock Production via Micromanipulation / Akira Onishi and Anthony C.F. Perry.
Part 8. Embryo Evaluation, Grading, and Assisted Hatching -- Pronuclear Scoring in Human In Vitro Fertilization / Lynette Scott -- Cumulative Morphological Assessment of Embryo Quality / Barry Behr and Aparna Hegde -- Metabolomics: The ViaMetrics-E Procedure for Assessing Embryo Viability / D. Sakkas, L. Botros, M. Henson, K. Judge and P. Roos -- Oxygen Consumption as an Indicator of Oocyte and Embryo Viability / Ana S. Lopes -- Gene Expression Changes During Human Early Embryo Development: New Applications for Embryo Selection / Samir Hamamah, Said Assou, Imene Boumela and Herve Dechaud -- Amino Acid Turnover as a Biomarker of Embryo Viability / Christine Leary, Danielle G. Smith, Henry J. Leese and Roger G. Sturmey -- Real-Time Embryo Monitoring Device for Embryo Selection / Gabor Vajta and Thorir Hardarson -- Assisted Hatching in IVF / Itziar Belil and Anna Veiga.
Part 9. Biopsy Procedures on Oocytes and Embryos -- Polar Body Biopsy / Markus Montag, Maria Koster, K. van der Ven and Hans van der Ven -- Cleavage-Stage Embryo Biopsy / Alan R. Thornhill -- Embryo Biopsy for PGD: Current Perspective / Steven J. McArthur, Don Leigh, Maria Traversa, James Marshall and Robert P.S. Jansen -- Microarrays and CGH for PGD of Chromosome Abnormalities and Gene Defects / Gary Harton and Santiago Munne.
Part 10. Cryopreservation -- Sperm Cryopreservation / Fabio Firmbach Pasqualotto, Eleonora Bedin Pasqualotto, Edson Borges and Ashok Agarwal -- Slow Freezing of Oocytes / Giovanni Coticchio and Lucia De Santis -- Vitrification: Research in Animal Models / Gabor Vajta -- Oocyte Vitrification / Ana Cobo -- Slow Freezing of Embryos / Liesl Nel-Themaat, Ching-Chien Chang, Thomas Elliott, Patricia Bernal and Graham Wright, et al. -- Vitrification of Embryos / Juergen Liebermann, Joe Conaghan, Zsolt Peter Nagy and Michael Tucker -- Directional Freezing of Reproductive Cells, Tissues, and Organs / Amir Arav -- Ovarian Tissue Cryopreservation / Jennifer L. Kulp, J. Ryan Martin and Pasquale Patrizio.
Part 11. Embryo Transfer -- Assessment of Uterine Receptivity / Hakan Cakmak and Hugh S. Taylor -- Single Embryo Transfer / Thorir Hardarson and Matts Wikland -- Ultrasound-Guided Embryo Transfer (Abdominal/Vaginal): An Evidence-Based Evaluation / Ahmed M. Abou-Setta -- Cumulus-Aided Embryo Transfer / Firuza R. Parikh, Nandkishor J. Naik, Dattatray J. Naik and Dhanajaya Kulkarni.
Part 12. Management and Regulation in the ART Laboratory -- Data Management in the ART Laboratory: Requirements and Solutions / Timothy Brown, Bruce R. Gilbert and Bruce R. Gilbert -- Regulation, Licensing, and Accreditation of the ART Laboratory / Doris Baker -- Legislation in the United Kingdom / Rachel Cutting -- Regulation, Licensing, and Accreditation of the ART Laboratory in Europe / Julius Hreinsson and Peter Sjoblom -- Regulation, Licensing, and Accreditation of ART Laboratories in India / B.N. Chakravarty and Rita Modi -- Troubleshooting in the Clinical Embryology Laboratory: The Art of Problem-Solving in ART / Kathryn J. Go, Jay C. Patel and Rick Dietz.
Part 13. Special Topics -- The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development / Jonathan Van Blerkom -- Nuclear and Cytoplasmic Transfer: Human Applications and Concerns / Josef Fulka and Helena Fulka -- Cytoskeletal Architecture of Human Oocytes with Focus on Centrosomes and Their Significant Role in Fertilization / Heide Schatten, Vanesa Y. Rawe and Qing-Yuan Sun -- Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence / Jonathan Van Blerkom.
Summary Annotation The Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is a unique, accessible title that provides a complete review of the most well-established and current diagnostic and treatment techniques comprising in vitro fertilization. Throughout the chapters, a uniform structure is employed, including a brief abstract, a keyword glossary, a step-by-step protocol of the laboratory procedures, several pages of expert commentary, key issues of clinical concern, and a list of references. The result is a readily accessible, high quality reference guide for reproductive endocrinologists, urologists, embryologists, biologists and research scientists. The Manual also offers an excellent description of novel procedures that will likely be employed in the near future. An indispensable resource for physicians and basic scientists, the Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is an invaluable reference and addition to the literature.
ISBN 9781441917805 (electronic bk.)
1441917802 (electronic bk.)
ISBN/ISSN 10.1007/978-1-4419-1780-5
OCLC # 792942305

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