| Table of Contents |
| | Contributors | |
| | Preface / Helmut Kettenmann, Rosemarie Grantyn | |
| Sect. 1 | Arrangement of Electrophysiological Setups | |
| | 1.1 | The Faraday Cage and Grounding Arrangements / C. Ohlemeyer, J. W. Meyer | 3 |
| | 1.2 | Infrared Videomicroscopy of Living Brain Slices / Hans-Ulrich Dodt | 6 |
| | 1.3 | Micromanipulation of Recording Electrodes / U. Sonnhof | 11 |
| | 1.4 | Recording Chambers - Slices / Helmut L. Haas, Dietrich Busselberg | 16 |
| | 1.5 | Microchamber for Electrophysiological Recording From Organotypic Slice Cultures / Scott M. Thompson, Beat H. Gahwiler | 20 |
| | 1.6 | Recording Chambers - Isolated and Perfused Mammalian Brain Preparations / M. Muhlethaler, M. Serafin | 23 |
| Sect. 2 | Preparations for Electrophysiological Experiments in Vitro | |
| | 2.1 | The Isolated Arterially Perfused Mammalian Eye / G. Niemeyer | 31 |
| | 2.2 | The Isolated Optic Nerve / Paula M. Orkand, Richard K. Orkand | 36 |
| | 2.3 | Hippocampal Slices / Ulrich Misgeld | 41 |
| | 2.4 | The Neocortical Slice / R. A. Deisz | 45 |
| | 2.5 | Hypothalamus In Vitro / Helmut L. Haas | 51 |
| | 2.6 | Cerebellar Slices for Patch Clamp Recording / M. Kano, A. Konnerth | 54 |
| | 2.7 | Organotypic Hippocampal Slice Cultures / Scott M. Thompson, Beat Gahwiler | 58 |
| | 2.8 | Organotypic Cocultures of Embryotic Rat Spinal Cord, Sensory Ganglia, and Skeletal Muscle / Hans-R. Luscher, C. Spenger | 62 |
| | 2.9 | Chronic Electrical Stimulation of Multicompartment Cell Cultures / R. Douglas Fields, Chang Yu, Elaine A. Neale, Phillip G. Nelson | 67 |
| | 2.10 | Freshly Dissociated and Cultured Rodent Retinal Ganglion Cells / Stuart A. Lipton | 77 |
| | 2.11 | Easy Identification of Dissociated Rat Retinal Ganglion Cells by a Size Criterion / Rosemarie Grantyn, E. Korenbaum | 84 |
| | 2.12 | Noninvasive Recording From Sensory Cilia of Olfactory Receptor Neurons Maintained In Situ in the Isolated Amphibian Olfactory Mucosa / Stephan Frings, Bernd Lindemann | 88 |
| | 2.13 | Synapfoneurosomes / Diana L. Kunze, John A. Drewe | 93 |
| | 2.14 | The Tissue Print Method / B. A. Barres | 96 |
| | 2.15 | Preparation of Astrocytes: Primary Culture and Acute Isolation / B. A. MacVicar, D. D. Fraser, S. Duffy | 101 |
| | 2.16 | Cryopreserved Human Neurons / Mark P. Mattson, Bin Cheng | 105 |
| | 2.17 | Neuroblastoma Cells / Georg Reiser | 111 |
| | 2.18 | Transiently Transfected Mammalian Cells for Studies of Recombinant Neurotransmitter Receptors / Todd A. Verdoom | 117 |
| | 2.19 | Xenopus Oocytes for Two-Electrode and Patch Clamp Recording / Walter Stuhmer, Heinrich Terlau, Stefan H. Heinemann | 121 |
| Sect. 3 | Electrical and Chemical Stimulation Techniques | |
| | 3.1 | Bath Perfusion of Slices With Drugs / Helmut L. Haas, Sebastian Hartter, Birgit Hermes, Christoph Hiemke | 129 |
| | 3.2 | Droplet Application of Agonists / Ulrich Misgeld | 132 |
| | 3.3 | U-Tube Drug Application / Joachim Bormann | 136 |
| | 3.4 | High-Speed Application With a Piezo Drive / J. Dudel, C. Franke, H. Hatt | 141 |
| | 3.5 | Spatially Selective Superfusion / K. Gottmann, K. Kraszewski | 146 |
| | 3.6 | Osmotic Stimulation of Presynaptic Terminals / John M. Bekkers, Charles F. Stevens | 150 |
| | 3.7 | Concentration Clamp / Norio Akaike | 155 |
| | 3.8 | Flash Photolysis / Sergej L. Mironov | 159 |
| | 3.9 | Recording Solutions for Isolating Specific Ionic Channel Currents / D. Swandulla, R. H. Chow | 164 |
| Sect. 4 | Electrodes for Recording | |
| | 4.1 | Extracellular Reference Electrodes / Francisco J. Alvarez-Leefmans | 171 |
| | 4.2 | Intracellular Microelectrodes / Christian M. Muller | 183 |
| | 4.3 | Suction Electrode Recording From Nerves and Fiber Tracts / Peter K. Stys | 189 |
| | 4.4 | Electrode Arrays for Extracellular Recording / Eva Sykova | 195 |
| | 4.5 | Arrays for Microiontophoresis / W. Zieglgansberger | 200 |
| | 4.6 | Production and Calibration of Ion-Sensitive Microelectrodes / U. Heinemann, J. Arens | 206 |
| | 4.7 | Intracellular Reference Electrodes / Francisco J. Alvarez-Leefmans | 213 |
| | 4.8 | Ion-Selective Membrane (ISM) Phases for Microelectrodes / Wilhelm Simon, Ursula Spichiger-Keller | 221 |
| | 4.9 | Calibration of Ion-Selective Microelectrodes / Jonathan A. Coles | 228 |
| | 4.10 | Fabrication of Patch Clamp Pipettes / A. Cavalie, Rosemarie Grantyn, H. D. Lux | 235 |
| | 4.11 | Intracellular Perfusion of Patch Electrodes / Jurgen Hescheler, Massaki Kameyama, Roland Speicher | 241 |
| Sect. 5 | Recording Modes | |
| | 5.1 | Extra- and Intracellular Voltage Recording in the Slice / Christian M. Muller | 249 |
| | 5.2 | Voltage Clamp Recording / I. D. Dietzel, D. Bruns, H.-R. Polder, H. D. Lux | 256 |
| | 5.3 | Single-Electrode Voltage Clamp / Stephen J. Redman | 263 |
| | 5.4 | Loose Patch Recording / Walter Stuhmer | 271 |
| | 5.5 | The Nystatin Method of Whole-Cell Patch Clamp Recording / Rosemarie Grantyn, K. Kraszewski, S. Richthof, Helmut Kettenmann | 274 |
| | 5.6 | Cell-Attached Patch Clamp / Meyer B. Jackson | 279 |
| | 5.7 | Cell-Free Patch Clamp / O. P. Hamill | 284 |
| | 5.8 | Double Whole-Cell Patch Clamp Technique / Hans-Albert Kolb | 289 |
| Sect. 6 | Dye and Substance Injection | |
| | 6.1 | HRP Staining With Recording Electrodes / Elaine A. Neale, Phillip G. Nelson | 299 |
| | 6.2 | Intracellular Recording and Labeling With Biocytin-Filled Electrodes / William E. Armstrong | 304 |
| | 6.3 | Application of Lucifer Yellow as Intracellular Marker in Electrophysiology / Gabriela von Blankenfeld, Helmut Kettenmann | 309 |
| | 6.4 | Quantitative Microinjection / Christian Hahnel | 316 |
| Sect. 7 | Special Applications | |
| | 7.1 | Pair Recording: Quantitative Reconstruction of a Reflex Pathway / Rosemarie Grantyn | 323 |
| | 7.2 | Pair Recording: Quantal Analysis of GABAergic Synaptic Transmission / K. Kraszewski, Rosemarie Grantyn | 330 |
| | 7.3 | Cell-Cell Coupling Demonstrated by Intracellular Injection of the Fluorescent Dye Lucifer Yellow / Bruce R. Ransom, Harald Sontheimer | 336 |
| | 7.4 | Use of Photo Bleaching to Study Gap Junctions / Harald Sontheimer | 343 |
| | 7.5 | Microsuction Assay of Cell-Cell Adhesion in Culture / Abraham Brown, Mu-Ming Poo | 349 |
| | 7.6 | Moving Patch Method for Measurement of Transmitter Release / Steven H. Young, Mu-Ming Poo | 354 |
| | 7.7 | Measurement of Cell Membrane Capacitance / Reinhold Penner | 358 |
| | 7.8 | Electrophysiological Measurement of Volume Changes in Neurons, Glial Cells and Muscle Fibers In Situ / Klaus Ballanyi, Michael Strupp, Peter Grafe | 363 |
| | 7.9 | Measurement of Extracellular Space / Charles Nicholson | 367 |
| | 7.10 | Measurement of Ion Activity With Selective Microelectrodes / Roger C. Thomas | 373 |
| | 7.11 | High Time Resolution Imaging of Calcium Transients With a CCD Camera / William N. Ross, Nechama Lasser-Ross | 378 |
| Sect. 8 | Storage of Data | |
| | 8.1 | Visualization and Storage of Electrophysiological Data / Helmut Kettenmann | 385 |
| | 8.2 | Converting Analog to Digital Data - Concepts and Pitfalls / Helmut Kettenmann | 389 |
| | 8.3 | Components of a Computer System for Electrophysiological Research / Helmut Kettenmann | 392 |
| Sect. 9 | Appendixes | |
| | 9.1 | Recording on a Small Budget / Inngard D. Dietzel, Vilem Bulka, Helmut Wintersberger | 399 |
| | 9.2 | Selection of Preparations for Application in the Pharmaceutical Industry / Marion Wienrich | 403 |
| 9.3 | List of Technical Equipment | 407 |
| 9.4 | List of Suppliers | 411 |
| | Index | 427 |
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