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Title Hepatitis C protocols / edited by Johnson Yiu-Nam Lau ; forewords by T. Jake Liang [and others].
Imprint Totowa, N.J. : Humana Press, 1998.

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Series Methods in molecular medicine ; 19
Methods in molecular medicine ; 19.
Subject Hepatitis C virus -- Research -- Methodology.
Hepatitis C Antibodies.
Hepatitis C Antigens.
Hepatitis C.
Hepatitis, Viral, Human.
Hepatitis Antibodies.
Flaviviridae Infections.
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Hepatitis Antigens.
Biological Science Disciplines.
Antibodies, Viral.
Virus Diseases.
RNA Virus Infections.
Antigens, Viral.
Liver Diseases.
Natural Science Disciplines.
Digestive System Diseases.
Biological Factors.
Disciplines and Occupations
Serum Globulins.
Chemicals and Drugs
Blood Proteins.
Amino Acids, Peptides, and Proteins
Alt Name Lau, Johnson Y. N. (Johnson Yiu-Nam)
Description 1 online resource (xxi, 621 pages) : illustrations
Bibliography Note Includes bibliographical references and index.
Contents Detection of anti-GOR antibodies in HCV infection / M.P. Manns and G. Michel -- Confirmation of HCV antibodies by the line immunoassay INNO-LIA HCV Ab III / G. Maertens [and others] -- Detection of HCV RNA in serum by reverse transcriptase-PCR and radiolabeled liquid hybridization / R.L. Hodinka -- Detection of HCV RNA in serum by reverse transcription polymerase chain reaction (RT-PCR) / K. Qian -- The AMPLICOR HCV tests for the detection and quantitation of serum or plasma HCV RNA / K.A. Gutekunst [and others] -- Quantification of HCV RNA in clinical specimens by branched DNA (bDNA) technology / J.C. Wilber and M.S. Urdea -- Quality control of the polymerase chain reaction / M. Rosenstraus [and others] -- Preparation of genotype-specific HCV RNA transcripts for assessing HCV detection and quantification assays / J.J. Detmer [and others] -- Detection of HCV RNA in formalin-fixed, paraffin-embedded liver tissue by RT-PCR / J.Y.N. Lau -- Quantification of HCV RNA in liver tissue by bDNA assay / P.J. Dailey [and others] -- Hepatitis C virus : types, subtypes, and beyond / D.B. Smith and P. Simmonds -- Molecular evolutionary analysis : its application in the study of hepatitis C virus / M. Mizokami and J.Y. Lau -- Genotyping by type-specific primers that can type HCV types 1-6 / T. Ohno and M. Mizokami -- Genotyping hepatitis C virus by type-specific primers for PCR based on NS5 region / K. Chayama -- Determination of HCV genotypes by RFLP / F. Davidson and P. Simmonds -- HCV genotyping by the line probe assay INNO-LiPA HCV II / G. Maertens and L. Stuyver --Serological genotyping using synthetic peptides derived from the NS4 region / L.E. Prescott and P. Simmonds -- Determination of HCV quasispecies by cloning and sequencing / M. Mizokami and T. Ohno -- Detection of hepatitis C virus quasispecies heterogeneity by single-strand conformational polymorphism / R.P. Gonzalez-Peralta -- Hepatitis C virus heteroduplex tracking assay : application to genotype determination, quasispecies analysis, and molecular evolution studies / A.J. Weiner [and others] -- In situ detection of HCV : an overview / F. Negro and J.Y.N. Lau -- In situ detection of hepatitis C viral antigens / R.P. Gonzalez-Peralta and K. Krawczynski -- In situ hybridization and the detection and localization of HCV RNA / F. Negro, D. Pacchioni and G. Bussolati -- The in situ detection of PCR-amplified hepatitis C RNA / G.J. Nuovo and M.L. Alfieri -- Immunoelectron microscopic characterization of HCV / S. Watanabe, M. Kaito and M. Kohara -- Cloning and assembly of complex libraries of full-length HCV cDNA clones / A.A. Kolykhalov, K.E. Reed and C.M. Rice -- Use of the vaccinia virus/T7 expression system for studying HCV protein processing / E.V. Agapov, K.E. Reed and C.M. Rice -- Use of a discistronic vector for the quantitation of HCV IRES activity / J.A. Feller and J.Y.N. Lau -- Expression and dimerization of hepatitis C virus core protein in E. coli / S.Y. Lo and J.H. Ou -- Expression and characterization of the HCV NS2 protease / K.E. Reed and C.M. Rice -- Expression and characterization of HCV NS3 protease / E. D'Souza [and others] -- Expression and characterization of the HCV NS3 helicase domain / F. Preugschat [and others] -- Hepatitis C virus RNA-dependent RNA polymerase (NS5B polymerase) / C.H. Hagedorn -- Detection and molecular cloning of the extreme 3'-end of HCV / T. Tanaka and and K. Shimotohno -- In vitro stability of hepatitis C virus RNA / J.W.S. Fang -- Methods for the study of sequence-specific binding of proteins to the HCV RNA genome / L.M. Kaplan and R.T. Chung -- Role of immune response in HCV / M. Peters -- Determination of hepatitis C virus-specific CD4+ T-cell activity in PBMC / J.T. Gerlach, H. Diepolder and G.R. Pape -- Measurement of HCV-specific CD8+ cytotoxic T-cell activities in the peripheral blood by europium release assay / M. Imawari -- Determination of HCV-specific bulk CD8+ activity in liver / D.R. Nelson -- Characterization of HCV-specific cytotoxic T-lymphocytes from liver tissue / M.J. Koziel -- Production of human monoclonal antibodies to hepatitis C virus and their characterization / M.U. Mondelli and A. Cerino -- Specific detection of negative strand RNA of hepatitis C virus using chemical RNA modification / T. Gunji and K. Shimotohno -- Strand-specific rTth RT-PCR for the analysis of HCV replication / R.E. Lanford and D. Chavez -- Cell culture systems for the detection of HCV infection / Y.K. Shimizu and H. Yoshikura -- Replication and detection of hepatitis C virus in liver-derived cell lines / R.P. Gonzalez-Peralta -- Primary human hepatocyte culture for the study of HCV / J.F. O'Connell [and others] -- A cultivation method for highly differentiated primary chimpanzee hepatocytes permissive for hepatitis C virus replication / R.E. Lanford and L. Estlack -- Construction of recombinant vaccinia virus expressing HCV genes / J.A. Feller -- Construction of recombinant adeno-associated virus (AAV) / M. Reiser and S. Zolotukhin -- Production of replication-deficient adenovirus recombinants / G.W.G. Wilkinson, C. Rickards and B.E. Clarke -- Generation of recombinant herpes simplex virus ampiicons / Z. Hong and A.D. Kwong -- Construction of recombinant sindbis-based expression vectors for the study of HCV genes and their products / B.D. Lindenbach, I. Frolov and C.M. Rice -- The chimpanzee model : contributions and considerations / E. Muchmore -- Liver transplantation as a model to study hepatitis C virus infection / H.M. Hassoba and T.L. Wright.
Note Print version record.
Access Use copy Restrictions unspecified star
Reproduction Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010.
System Details Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Note digitized 2010 HathiTrust Digital Library committed to preserve pda
Summary In Hepatitis C Protocols, Johnson YN Lau and his expert collaborators present cutting-edge techniques for study of the hepatitis C virus infection and its disease, hepatitis C, which afflict an estimated 170 million people worldwide. The methods range from the detection of anti-HCV in serum and HCV in liver tissue, to HCV genotyping and the detection and quantification of HCV in serum. In addition to these key protocols, the authoritative contributors also evaluate recombinant proteins in immunological test systems, including the identification of important antigenic epitopes, antibodies, and recombinant antigens. Molecular and immunological techniques to study this virus and its infection, as well as animal models and recombinant virus technology commonly used to express hepatitis C viral proteins, are also developed. Hepatitis C Protocols summarizes the state-of-the-art in today's research and understanding of the virology of hepatitis C virus. The methods and systems detailed in this book will play a key role in the development of more effective antivirals, in the development of vaccines, and in a deeper understanding of viral replication strategies, viron structure, and the mechanisms of viron assembly and release. A valuable new standard resource for all researchers and clinicians, Hepatitis C Protocols leads the way toward improved prevention, diagnosis, and treatment of viral hepatitis C.
Note English.
ISBN 9781592592609 (electronic bk.)
1592592600 (Springer e-ISBN)
0585235651 (NetLibrary e-ISBN)
9780585235653 (NetLibrary e-ISBN)
0896035212 (alk. paper)
9780896035218 (alk. paper)
ISBN/ISSN 10.1385/0896035212.
OCLC # 45729313
Additional Format Print version: Hepatitis C protocols. Totowa, N.J. : Humana Press, 1998 0896035212 (DLC) 98024896 (OCoLC)39131266
Table of Contents
 Foreword / T. Jake Liang 
 Foreword / Gary L. Davis 
 Foreword / Michael Houghton, Qui-Lim Choo, George Kuo 
Pt. IDetection of Anti-HCV in Serum1
 1Detection of Anti-GOR Antibodies in HCV Infection / Michael P. Manns, Gerd Michel3
 2Confirmation of HCV Antibodies by the Line Immunoassay INNO-LIA HCV Ab III / Geert Maertens, Filip Dekeyser, Anja Van Geel [et al.]11
Pt. IIDetection and Quantitation of HCV in Serum27
 3Detection of HCV RNA in Serum by Reverse Transcriptase-PCR and Radiolabeled Liquid Hybridization / Richard L. Hodinka29
 4Detection of HCV RNA in Serum by Reverse Transcription Polymerase Chain Reaction (RT-PCR) / KePing Qian47
 5The AMPLICOR HCV Tests for the Detection and Quantitation of Serum or Plasma HCV RNA / Karen A. Gutekunst, Joanne P. Spadoro, Elizabeth A. Dragon [et al.]55
 6Quantification of HCV RNA in Clinical Specimens by Branched DNA (bDNA) Technology / Judith C. Wilber, Mickey S. Urdea71
 7Quality Control of the Polymerase Chain Reaction / Maurice Rosenstraus, Joanne P. Spadoro, Diane McGovern-Wolfe [et al.]79
 8Preparation of Genotype-Specific HCV RNA Transcripts for Assessing HCV Detection and Quantification Assays / Jill J. Detmer, Janice A. Kolberg, Crystle L. K. Zayati [et al.]99
Pt. IIIDetection of HCV in Liver Tissue113
 9Detection of HCV RNA in Formalin-Fixed, Paraffin-Embedded Liver Tissue by RT-PCR / Johnson Y. N. Lau115
 10Quantification of HCV RNA in Liver Tissue by bDNA Assay / Peter J. Dailey, Mark L. Collins, Mickey S. Urdea [et al.]119
Pt. IVHCV Genotypes131
 11Hepatitis C Virus: Types, Subtypes, and Beyond / Donald B. Smith, Peter Simmonds133
 12Molecular Evolutionary Analysis: Its Application in the Study of Hepatitis C Virus / Masashi Mizokami, Johnson Y. N. Lau147
 13Genotyping by Type-Specific Primers That Can Type HCV Types 1-6 / Tomoyoshi Ohno, Masashi Mizokami159
 14Genotyping Hepatitis C Virus by Type-Specific Primers for PCR Based on NS5 Region / Kazuaki Chayama165
 15Determination of HCV Genotypes by RFLP / Fiona Davidson, Peter Simmonds175
 16HCV Genotyping by the Line Probe Assay INNO-LiPA HCV II / Geert Maertens, Lieven Stuyver183
 17Serological Genotyping Using Synthetic Peptides Derived from the NS4 Region / Linda E. Prescott, Peter Simmonds199
 18Determination of HCV Quasispecies by Cloning and Sequencing / Masashi Mizokami, Tomoyoshi Ohno207
 19Detection of Hepatitis C Virus Quasispecies Heterogeneity by Single-Strand Conformational Polymorphism / Regino P. Gonzalez-Peraita213
 20Hepatitis C Virus Heteroduplex Tracking Assay: Application to Genotype Determination, Quasispecies Analysis, and Molecular Evolution Studies / Amy J. Weiner, Piero L. Calvo, Joe Kansopon [et al.]221
Pt. VDetection of HCV In Situ235
 21In Situ Detection of HCV: An Overview / Francesco Negro, Johnson Y. N. Lau237
 22In Situ Detection of Hepatitis C Viral Antigens / Regino P. Gonzalez-Peralta, Krzysztof Krawczynski249
 23In Situ Hybridization and the Detection and Localization of HCV RNA / Francesco Negro, Donatella Pacchioni, Gianni Bussolati257
 24The In Situ Detection of PCR-Amplified Hepatitis C RNA / Gerard J. Nuovo, Maria Lynn Alfieri263
 25Immunoelectron Microscopic Characterization of HCV / Shozo Watanabe, Masahiko Kaito, Michinori Kohara279
Pt. VIMolecular Biologic Characterization287
 26Cloning and Assembly of Complex Libraries of Full-Length HCV cDNA Clones / Alexander A. Kolykhalov, Karen E. Reed, Charles M. Rice289
 27Use of the Vaccinia Virus/T7 Expression System for Studying HCV Protein Processing / Eugene V. Agapov, Karen E. Reed, Charles M. Rice303
 28Use of a Discistronic Vector for the Quantitation of HCV IRES Activity / Joyce A. Feller, Johnson Y. N. Lau315
 29Expression and Dimerization of Hepatitis C Virus Core Protein in E. coli / Shih-Yen Lo, Jing-Hsiung Ou325
 30Expression and Characterization of the HCV NS2 Protease / Karen E. Reed, Charles M. Rice331
 31Expression and Characterization of HCV NS3 Protease / Eric D'Souza, Norman Gray, Malcolm Ellis [et al.]343
 32Expression and Characterization of the HCV NS3 Helicase Domain / Frank Preugschat, Mary H. Hanlon, Martin J. Rink [et al.]353
 33Hepatitis C Virus RNA-Dependent RNA Polymerase (NS5B Polymerase) / Curt H. Hagedorn365
 34Detection and Molecular Cloning of the Extreme 3'-End of HCV / Torahiko Tanaka, Kunitada Shimotohno373
 35In Vitro Stability of Hepatitis C Virus RNA / Jane W. S. Fang381
 36Methods for the Study of Sequence-Specific Binding of Proteins to the HCV RNA Genome / Lee M. Kaplan, Raymond T. Chung385
Pt. VIIHCV-Specific Immunologic Response405
 37Role of Immune Response in HCV / Marion Peters407
 38Determination of Hepatitis C Virus-Specific CD4[superscript +] T-Cell Activity in PBMC / J. Tilman Gerlach, Helmut Diepolder, Gerd R. Pape413
 39Measurement of HCV-Specific CD8[superscript +] Cytotoxic T-Cell Activities in the Peripheral Blood by Europium Release Assay / Michio Imawari423
 40Determination of HCV-Specific Bulk CD8[superscript +] Activity in Liver / David R. Nelson431
 41Characterization of HCV-Specific Cytotoxic T-Lymphocytes from Liver Tissue / Margaret James Koziel439
 42Production of Human Monoclonal Antibodies to Hepatitis C Virus and Their Characterization / Mario U. Mondelli, Antonella Cerino451
Pt. VIIIIn Vitro Culture Model463
 43Specific Detection of Negative Strand RNA of Hepatitis C Virus Using Chemical RNA Modification / Toshiaki Gunji, Kunitada Shimotohno465
 44Strand-Specific rTth RT-PCR for the Analysis of HCV Replication / Robert E. Lanford, Deborah Chavez471
 45Cell Culture Systems for the Detection of HCV Infection / Yohko K. Shimizu, Hiroshi Yoshikura483
 46Replication and Detection of Hepatitis C Virus in Liver-Derived Cell Lines / Regino P. Gonzalez-Peralta489
 47Primary Human Hepatocyte Culture for the Study of HCV / John F. O'Connell, Stuart Cox, Peter Buontempo [et al.]495
 48A Cultivation Method for Highly Differentiated Primary Chimpanzee Hepatocytes Permissive for Hepatitis C Virus Replication / Robert E. Lanford, Larry Estlack501
Pt. IXConstruction of Recombinant Viral Vectors for the Expression of HCV Genes517
 49Construction of Recombinant Vaccinia Virus Expressing HCV Genes / Joyce A. Feller519
 50Construction of Recombinant Adeno-Associated Virus (AAV) / Markus Reiser, Sergei Zolotukhin533
 51Production of Replication-Deficient Adenovirus Recombinants / Gavin W. G. Wilkinson, Carole Rickards, Berwyn E. Clarke539
 52Generation of Recombinant Herpes Simplex Virus Amplicons / Zhi Hong, Ann D. Kwong553
 53Construction of Recombinant Sindbis-Based Expression Vectors for the Study of HCV Genes and Their Products / Brett D. Lindenbach, Ilya Frolov, Charles M. Rice565
Pt. XIn Vivo Models575
 54The Chimpanzee Model: Contributions and Considerations / Elizabeth Muchmore577
 55Liver Transplantation as a Model to Study Hepatitis C Virus Infection / Howayda M. Hassoba, Teresa L. Wright589

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