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Title PCR for clinical microbiology : an Australian and international perspective / edited by Margret Schuller [and others].
Imprint Dordrecht ; London : Springer, 2010.

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Subject Polymerase chain reaction -- Diagnostic use -- Handbooks, manuals, etc.
Diagnostic microbiology -- Handbooks, manuals, etc.
Polymerase Chain Reaction -- statistics & numerical data.
Diagnosis.
Alt Name Schuller, Margret.
Description 1 online resource
Bibliography Note Includes bibliographical references and index.
Summary Not another textbook, but a valuable tool for doctors and microbiologists wanting to know how to set up a PCR diagnostic microbiology laboratory according to current regulatory standards and perform assays supplied with patient clinical diagnostic criteria and easy to follow protocols. Whether laboratories are using commercial kits or in-house methods developed in their own laboratories or adopted from published methods, all clinical microbiology laboratories need to be able to understand, critically evaluate, perform and interpret these tests according to rigorous and clinically appropriate standards and international guidelines. The cost and effort of development and evaluation of in-house tests is considerable and many laboratories do not have the resources to do so. This compendium is a vehicle to improve and maintain the clinical relevance and high quality of diagnostic PCR. It is a unique collection of; guidelines for PCR laboratory set up and quality control, test selection criteria, methods and detailed step by step protocols for a diagnostic assays in the field of molecular microbiology. The structure of the book provides the PCR fundamentals and describes the clinical aspects and diagnosis of infectious disease. This is followed by protocols divided into; bacteria, virus, fungi and parasites, and susceptibility screens. The inclusion of medical criteria and interpretation adds value to the compendium and benefits clinicians, scientists, researchers and students of clinical diagnostic microbiology.
Access Licensed electronic resource available to Carleton faculty, staff, students and walk-in users. Not available to alumni.
Contents Foreword -- Contents -- Contributors -- Abbreviations -- Introduction -- References -- Part I PCR Fundamentals -- 1 PCR Basics -- 1.1 Introduction -- 1.2 Facility Layout, Airflow and Workflow -- 1.3 Good Molecular Biology Laboratory Practices -- References -- 2 PCR Methodology -- 2.1 Nucleic Acid Extraction, Purification and Storage -- 2.2 Conventional PCR -- 2.2.1 Introduction -- 2.2.2 Components of PCR -- 2.2.3 PCR Amplification and Product Detection -- 2.2.4 Conventional PCR in the Diagnostic Laboratory -- 2.2.5 Limitations of Conventional PCR -- 2.2.6 Summary -- 2.3 Real-Time PCR.
2.3.1 Introduction -- 2.3.2 Real-Time PCR Technology -- 2.3.3 Real-Time PCR in the Diagnostic Laboratory -- 2.3.4 Instrumentation for Real-Time PCR -- 2.3.5 Considerations in the Use of Real-Time PCR -- 2.3.6 Summary -- 2.4 Quantitative PCR -- 2.4.1 Introduction -- 2.4.2 PCR Kinetics and Q-PCR -- 2.4.3 Absolute Quantification with External Standards -- 2.4.4 Absolute Quantification Using Internal Controls -- 2.4.5 Detection Formats -- 2.4.5.1 Intercalating Dyes -- 2.4.5.2 Sequence-Specific Fluorescent Dyes -- 2.4.6 Clinical Use -- 2.4.7 Conclusion -- 2.5 Multiplex PCR in Diagnostic Microbiology.
2.5.1 Introduction -- 2.5.2 Multiplex Technologies -- 2.5.3 Practical Applications -- 2.6 Molecular Subtyping -- 2.6.1 Introduction -- 2.6.2 Why Bacterial Typing Is Performed -- 2.6.3 Typing Methods Based on Phenotype -- 2.6.4 Typing Methods Based on Electrophoresis of DNA Fragments Derived from the Whole Genome -- 2.6.5 Typing Methods Based upon Known Polymorphic Genes or Sites -- 2.6.6 Projections of Future Developments -- References -- 3 Laboratory Accreditation Standards -- 3.1 Quality in the Medical Molecular Microbiology Laboratory -- 3.1.1 Part 1: Overview.
3.1.1.1 Quality and the Role of the Laboratory -- 3.1.1.2 Legislating for Quality -- 3.1.1.3 Quality and Standards -- 3.1.1.4 Quality and Regulation -- 3.1.2 Part 2: Specific Aspects Relating to Quality -- 3.1.2.1 Risk Management -- 3.1.2.2 Validation and Verification -- 3.1.2.3 Quality Assurance -- 3.1.2.4 Conclusion -- 3.1.3 Annex -- 3.1.3.1 Compendium of Standards and Guidelines for Medical Molecular Microbiological Laboratories -- 3.1.3.2 European Committee for Standardization -- 3.1.3.3 Clinical and Laboratory Standards Institute (CLSI) Standards.
3.2 Security Sensitive Biological Agents (SSBA) and Office of the Gene Technology Regulator (OGTR) Standards -- 3.2.1 Security Sensitive Biological Agents (SSBA) Regulatory Scheme -- 3.2.2 Regulation of Gene Technology -- Part II Clinical Aspects and Diagnosis of Infectious Disease -- 4 Meningitis and Encephalitis -- References -- 5 Respiratory Infections -- 5.1 Introduction -- 5.2 Clinical Aspects and Epidemiology -- 5.2.1 Clinical Presentation -- 5.2.2 Pathogenesis (Transmission, Incubation, Site of Infection) -- 5.2.3 Epidemiology (Frequency, Seasonality, Age Groups)
Note Print version record.
ISBN 9789048190393
9048190398
9789048190386
904819038X
OCLC # 663093681
Additional Format Print version: PCR for clinical microbiology. Dordrecht ; London : Springer, 2010 9789048190386 904819038X (OCoLC)551432070